dc.contributor.author | Ellefsen, Christiane Færestrand | |
dc.contributor.author | Lindstad, Linda | |
dc.contributor.author | Klau, Leesa Jane | |
dc.contributor.author | Aachmann, Finn Lillelund | |
dc.contributor.author | Hiorth, Marianne | |
dc.contributor.author | Samuelsen, Anne Berit C. | |
dc.date.accessioned | 2024-02-06T14:49:40Z | |
dc.date.available | 2024-02-06T14:49:40Z | |
dc.date.created | 2023-09-27T15:46:03Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Carbohydrate Polymers. 2023, 322 1-10. | en_US |
dc.identifier.issn | 0144-8617 | |
dc.identifier.uri | https://hdl.handle.net/11250/3116014 | |
dc.description.abstract | Fungal β-glucans have received a lot of interest due to their proinflammatory activity towards cells of the innate immune system. Although commonly described as (1➔3)-β-glucans with varying degree of (1➔6)-branching, the fungal β-glucans constitute a diverse polysaccharide class. In this study, the alkali-soluble β-glucans from the edible mushroom Pleurotus eryngii were extracted and characterized by GC, GC–MS and 2D NMR analyses. The extracts contain several structurally different polysaccharides, including a (1➔3)-β-d-glucan with single glucose units attached at O-6, and a (1➔6)-β-d-glucan, possibly branched at O-3. The immunomodulatory activities of the P. eryngii extracts were assessed by investigating their ability to bind to the receptor dectin-1, and their ability to induce production of the proinflammatory cytokines TNF-α, IL-6 and IL-1β in LPS-differentiated THP-1 cells. Although the samples were able to bind to the dectin-1a receptor, they did not induce production of significant levels of cytokines in the THP-1 cells. Positive controls of yeast-derived (1➔3)-β-d-glucans with branches at O-6 induced cytokine production in the cells. Thus, it appears that the P. eryngii β-glucans are unable to induce production of proinflammatory cytokines in LPS-differentiated THP-1 cells, despite being able to activate the human dectin-1a receptor. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier B. V. | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Investigation of the structural and immunomodulatory properties of alkali-soluble β-glucans from Pleurotus eryngii fruiting bodies | en_US |
dc.title.alternative | Investigation of the structural and immunomodulatory properties of alkali-soluble β-glucans from Pleurotus eryngii fruiting bodies | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 1-10 | en_US |
dc.source.volume | 322 | en_US |
dc.source.journal | Carbohydrate Polymers | en_US |
dc.identifier.doi | 10.1016/j.carbpol.2023.121367 | |
dc.identifier.cristin | 2179559 | |
dc.relation.project | Norges forskningsråd: 294946 | en_US |
dc.relation.project | Norges forskningsråd: 226244 | en_US |
dc.source.articlenumber | 121367 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |