dc.contributor.author | Maassen, Hanno | |
dc.contributor.author | Venema, Leonie H. | |
dc.contributor.author | Weiss, Marc G. | |
dc.contributor.author | Huijink, Tobias M. | |
dc.contributor.author | Hofker, H. Sijbrand | |
dc.contributor.author | Keller, Anna K. | |
dc.contributor.author | Mollnes, Tom Eirik | |
dc.contributor.author | Eijken, Marco | |
dc.contributor.author | Pischke, Soeren | |
dc.contributor.author | Jespersen, Bente | |
dc.contributor.author | van Goor, Harry | |
dc.contributor.author | Leuvenink, Henri G. D. | |
dc.date.accessioned | 2023-11-03T09:43:38Z | |
dc.date.available | 2023-11-03T09:43:38Z | |
dc.date.created | 2023-04-20T09:01:21Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Antioxidants. 2023, 12 (3), . | en_US |
dc.identifier.issn | 2076-3921 | |
dc.identifier.uri | https://hdl.handle.net/11250/3100463 | |
dc.description.abstract | Kidney extraction time has a detrimental effect on post-transplantation outcome. This study aims to improve the flush-out and potentially decrease ischemic injury by the addition of hydrogen sulphide (H2S) to the flush medium. Porcine kidneys (n = 22) were extracted during organ recovery surgery. Pigs underwent brain death induction or a Sham operation, resulting in four groups: donation after brain death (DBD) control, DBD H2S, non-DBD control, and non-DBD H2S. Directly after the abdominal flush, kidneys were extracted and flushed with or without H2S and stored for 13 h via static cold storage (SCS) +/− H2S before reperfusion on normothermic machine perfusion. Pro-inflammatory cytokines IL-1b and IL-8 were significantly lower in H2S treated DBD kidneys during NMP (p = 0.03). The non-DBD kidneys show superiority in renal function (creatinine clearance and FENa) compared to the DBD control group (p = 0.03 and p = 0.004). No differences were seen in perfusion parameters, injury markers and histological appearance. We found an overall trend of better renal function in the non-DBD kidneys compared to the DBD kidneys. The addition of H2S during the flush out and SCS resulted in a reduction in pro-inflammatory cytokines without affecting renal function or injury markers. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | MDPI | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | H2S-Enriched Flush out Does Not Increase Donor Organ Quality in a Porcine Kidney Perfusion Model | en_US |
dc.title.alternative | H<inf>2</inf>S-Enriched Flush out Does Not Increase Donor Organ Quality in a Porcine Kidney Perfusion Model | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 0 | en_US |
dc.source.volume | 12 | en_US |
dc.source.journal | Antioxidants | en_US |
dc.source.issue | 3 | en_US |
dc.identifier.doi | 10.3390/antiox12030749 | |
dc.identifier.cristin | 2141997 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |