dc.contributor.author | Holme, Jørn Andreas | |
dc.contributor.author | Låg, Marit | |
dc.contributor.author | Skuland, Tonje Schwach | |
dc.contributor.author | Parenicová, Martina | |
dc.contributor.author | Ciganek, Miroslav | |
dc.contributor.author | Penciková, Katerina | |
dc.contributor.author | Grytting, Vegard Sæter | |
dc.contributor.author | Neca, Jiri | |
dc.contributor.author | Øvrevik, Johan | |
dc.contributor.author | Mariussen, Espen | |
dc.contributor.author | Jørgensen, Rikke Bramming | |
dc.contributor.author | Refsnes, Magne Arnold | |
dc.contributor.author | Machala, Miroslav | |
dc.date.accessioned | 2023-07-21T12:50:35Z | |
dc.date.available | 2023-07-21T12:50:35Z | |
dc.date.created | 2023-05-30T09:49:16Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Toxicology in Vitro. 2023, 90 1-13. | |
dc.identifier.issn | 0887-2333 | |
dc.identifier.uri | https://hdl.handle.net/11250/3080694 | |
dc.description.abstract | The aims were to characterize the content of elements and polycyclic aromatic hydrocarbons (PAHs) in size-separated particulate matter (PM) sampled in a road tunnel, estimate the contribution of PAHs to the toxic potential, and measure the pro-inflammatory potential of PM samples and extracts with increasing polarity. Several elements/metals previously associated with cytokine responses were found. Based on PAHs levels and published PAHs potency, the calculated mutagenic and carcinogenic activities of size-separated samples were somewhat lower for coarse than fine and ultrafine PM. The AhR-activity of the corresponding PM extracts measured in an AhR-luciferase reporter model (human hepatocytes) were more similar. The highest AhR-activity was found in the neutral (parent and alkylated PAHs) and polar (oxy-PAHs) fractions, while the semi-polar fractions (mono-nitrated-PAHs) had only weak activity. The neutral and polar aromatic fractions from coarse and fine PM were also found to induce higher pro-inflammatory responses and CYP1A1 expression in human bronchial epithelial cells (HBEC3-KT) than the semi-polar fractions. Fine PM induced higher pro-inflammatory responses than coarse PM. AhR-inhibition reduced cytokine responses induced by parent PM and extracts of both size fractions. Contributors to the toxic potentials include PAHs and oxy-PAHs, but substantial contributions from other organic compounds and/or metals are likely. | |
dc.language.iso | eng | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | |
dc.title | Characterization of elements, PAHs, AhR-activity and pro-inflammatory responses of road tunnel-derived particulate matter in human hepatocyte-like and bronchial epithelial cells | |
dc.title.alternative | Characterization of elements, PAHs, AhR-activity and pro-inflammatory responses of road tunnel-derived particulate matter in human hepatocyte-like and bronchial epithelial cells | |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | |
dc.source.pagenumber | 1-13 | |
dc.source.volume | 90 | |
dc.source.journal | Toxicology in Vitro | |
dc.identifier.doi | 10.1016/j.tiv.2023.105611 | |
dc.identifier.cristin | 2150057 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |