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dc.contributor.authorSchiro, Lars Erik
dc.contributor.authorBauer, Ulrich Stefan
dc.contributor.authorSandvig, Axel
dc.contributor.authorSandvig, Ioanna
dc.date.accessioned2023-02-06T10:19:13Z
dc.date.available2023-02-06T10:19:13Z
dc.date.created2022-06-14T09:08:34Z
dc.date.issued2022
dc.identifier.issn2666-1667
dc.identifier.urihttps://hdl.handle.net/11250/3048511
dc.description.abstractHere, we present a unified protocol for the extraction, culture, and basic characterization of rat neural stem cells (NSCs) from all three canonical neurogenic niches in the brain and spinal cord. We describe tissue dissection and dissociation, cell culture, followed by EdU labeling and characterization of NSCs. By yielding considerable numbers of viable cells per animal, this protocol enables the establishment of substantial, long-term cell banks, thus offering cost and labor efficiency while significantly reducing the numbers of animals used.en_US
dc.language.isoengen_US
dc.publisherElsevier Scienceen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleIsolation and comparison of neural stem cells from the adult rat brain and spinal cord canonical neurogenic nichesen_US
dc.title.alternativeIsolation and comparison of neural stem cells from the adult rat brain and spinal cord canonical neurogenic nichesen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.volume3en_US
dc.source.journalSTAR Protocolsen_US
dc.source.issue2en_US
dc.identifier.doi10.1016/j.xpro.2022.101426
dc.identifier.cristin2031631
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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