dc.contributor.author | Hennig, Guido | |
dc.contributor.author | Haupka, Carsten | |
dc.contributor.author | Fernandes de Brito, Luciana | |
dc.contributor.author | Rückert, Christian | |
dc.contributor.author | Cahoreau, Edern | |
dc.contributor.author | Heux, Stéphanie | |
dc.contributor.author | Wendisch, Volker F. | |
dc.date.accessioned | 2022-12-08T09:31:33Z | |
dc.date.available | 2022-12-08T09:31:33Z | |
dc.date.created | 2021-03-20T17:45:09Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | International Journal of Molecular Sciences. 2020, 21:3617 (10), 1-16. | en_US |
dc.identifier.issn | 1661-6596 | |
dc.identifier.uri | https://hdl.handle.net/11250/3036676 | |
dc.description.abstract | Methanol is a sustainable substrate for biotechnology. In addition to natural methylotrophs, metabolic engineering has gained attention for transfer of methylotrophy. Here, we engineered Corynebacterium glutamicum for methanol-dependent growth with a sugar co-substrate. Heterologous expression of genes for methanol dehydrogenase from Bacillus methanolicus and of ribulose monophosphate pathway genes for hexulose phosphate synthase and isomerase from Bacillus subtilis enabled methanol-dependent growth of mutants carrying one of two independent metabolic cut-offs, i.e., either lacking ribose-5-phosphate isomerase or ribulose-5-phosphate epimerase. Whole genome sequencing of strains selected by adaptive laboratory evolution (ALE) for faster methanol-dependent growth was performed. Subsequently, three mutations were identified that caused improved methanol-dependent growth by (1) increased plasmid copy numbers, (2) enhanced riboflavin supply and (3) reduced formation of the methionine-analogue O-methyl-homoserine in the methanethiol pathway. Our findings serve as a foundation for the engineering of C. glutamicum to unleash the full potential of methanol as a carbon source in biotechnological processes. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | MDPI | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Methanol-essential growth of corynebacterium glutamicum: Adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway | en_US |
dc.title.alternative | Methanol-essential growth of corynebacterium glutamicum: Adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 1-16 | en_US |
dc.source.volume | 21:3617 | en_US |
dc.source.journal | International Journal of Molecular Sciences | en_US |
dc.source.issue | 10 | en_US |
dc.identifier.doi | 10.3390/ijms21103617 | |
dc.identifier.cristin | 1899625 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |