dc.contributor.author | Xie, Mingxu | |
dc.contributor.author | Li, Yu | |
dc.contributor.author | Olsen, Rolf Erik | |
dc.contributor.author | Ringø, Einar | |
dc.contributor.author | Yang, Yalin | |
dc.contributor.author | Zhang, Zhen | |
dc.contributor.author | Ran, Chao | |
dc.contributor.author | Zhou, Zhigang | |
dc.date.accessioned | 2022-11-29T16:33:15Z | |
dc.date.available | 2022-11-29T16:33:15Z | |
dc.date.created | 2022-08-30T13:35:24Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Frontiers in Immunology. 2022, 13 . | en_US |
dc.identifier.issn | 1664-3224 | |
dc.identifier.uri | https://hdl.handle.net/11250/3034836 | |
dc.description.abstract | Spring viremia of carp virus (SVCV) can cause high mortality of fish. The aim of this study was to investigate the effects of Lactobacillus rhamnosus GCC-3 exopolysaccharides (GCC-3 EPS) on zebrafish (Danio rerio) infected with SVCV and elucidate the underlying mechanisms. Zebrafish were fed with a control diet or diet supplemented with 0.5% and 1% of GCC-3 EPS for 2 weeks. The results showed that supplementation of GCC-3 EPS significantly improved the survival rate of zebrafish compared with the control group. In addition, dietary 0.5% and 1% GCC-3 EPS significantly up-regulated the expression of genes related to type I interferon (IFN) antiviral immunity. Consistent with in vivo results, GCC-3 EPS significantly inhibited SVCV replication in zebrafish embryonic fibroblast (ZF4) cells while significantly increased the expression of type I IFN signaling pathway related genes. Furthermore, knocking down TANK-binding kinase 1 significantly blocked the antiviral effect of GCC-3 EPS. Dietary GCC-3 EPS improved gut microbiota, and the culture supernatant of GCC-3 EPS-associated microbiota significantly inhibited SVCV replication in ZF4 cells compared with the control-microbiota counterpart. In conclusion, our results indicate that dietary GCC-3 EPS can improve the resistance of zebrafish against SVCV infection, and the mechanism may involve enhanced type I interferon signaling. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Frontiers Media | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Dietary supplementation of exopolysaccharides from Lactobacillus rhamnosus GCC-3 improved the resistance of zebrafish against spring viremia of carp virus infection | en_US |
dc.title.alternative | Dietary supplementation of exopolysaccharides from Lactobacillus rhamnosus GCC-3 improved the resistance of zebrafish against spring viremia of carp virus infection | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 12 | en_US |
dc.source.volume | 13 | en_US |
dc.source.journal | Frontiers in Immunology | en_US |
dc.identifier.doi | 10.3389/fimmu.2022.968348 | |
dc.identifier.cristin | 2047219 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |