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dc.contributor.authorZong, Weijian
dc.contributor.authorObenhaus, Horst-Andreas
dc.contributor.authorSkytøen, Emilie
dc.contributor.authorEneqvist, Hanna
dc.contributor.authorde Jong, Nienke Laura
dc.contributor.authorVale, Ruben Duque do
dc.contributor.authorRodrigues Jorge, Marina
dc.contributor.authorMoser, May-Britt
dc.contributor.authorMoser, Edvard Ingjald
dc.date.accessioned2022-10-03T06:09:33Z
dc.date.available2022-10-03T06:09:33Z
dc.date.created2022-04-09T15:15:49Z
dc.date.issued2022
dc.identifier.citationCell. 2022, 185 (7), 1240-1256.e30.en_US
dc.identifier.issn0092-8674
dc.identifier.urihttps://hdl.handle.net/11250/3023142
dc.description.abstractWe developed a miniaturized two-photon microscope (MINI2P) for fast, high-resolution, multiplane calcium imaging of over 1,000 neurons at a time in freely moving mice. With a microscope weight below 3 g and a highly flexible connection cable, MINI2P allowed stable imaging with no impediment of behavior in a variety of assays compared to untethered, unimplanted animals. The improved cell yield was achieved through a optical system design featuring an enlarged field of view (FOV) and a microtunable lens with increased z-scanning range and speed that allows fast and stable imaging of multiple interleaved planes, as well as 3D functional imaging. Successive imaging across multiple, adjacent FOVs enabled recordings from more than 10,000 neurons in the same animal. Large-scale proof-of-principle data were obtained from cell populations in visual cortex, medial entorhinal cortex, and hippocampus, revealing spatial tuning of cells in all areas.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleLarge-scale two-photon calcium imaging in freely moving miceen_US
dc.title.alternativeLarge-scale two-photon calcium imaging in freely moving miceen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.source.pagenumber1240-1256.e30en_US
dc.source.volume185en_US
dc.source.journalCellen_US
dc.source.issue7en_US
dc.identifier.doi10.1016/j.cell.2022.02.017
dc.identifier.cristin2016371
cristin.ispublishedtrue
cristin.fulltextpostprint
cristin.qualitycode2


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal