Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay

Nilsson, Per; Pettersen, Kristin; Oppermann, Martin; Skjeflo, Espen Waage; Fure, Hilde; Christiansen, Dorte; Mollnes, Tom Eirik
Peer reviewed, Journal article
Accepted version
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https://hdl.handle.net/11250/2992870
Utgivelsesdato
2021
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Originalversjon
Methods in molecular biology. 2021, 2227 51-59.   10.1007/978-1-0716-1016-9_5
Sammendrag
Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3.
Utgiver
Springer
Tidsskrift
Methods in molecular biology
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This is the authors' accepted manuscript to an article published by Springer.