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dc.contributor.authorBjørnetrø, Tonje
dc.contributor.authorSteffensen, Lilly Alice
dc.contributor.authorVestad, Beate
dc.contributor.authorBrusletto, Berit Sletbakk
dc.contributor.authorOlstad, Ole Kristoffer
dc.contributor.authorSiebke, Anne-Marie
dc.contributor.authorAass, Hans Christian Dalsbotten
dc.contributor.authorFoss, Kari Bente
dc.contributor.authorLlorente, Alicia
dc.contributor.authorBøe, Stig Ove
dc.contributor.authorLång, Anna Ulrika
dc.contributor.authorSamiappan, Rampradeep
dc.contributor.authorRedalen, Kathrine
dc.contributor.authorØvstebø, Reidun
dc.contributor.authorRee, Anne Hansen
dc.identifier.citationFEBS Open Bio. 2021, 11 (3), 724-740.en_US
dc.description.abstractExtracellular vesicles (EVs) released by tumor cells can directly or indirectly modulate the phenotype and function of the immune cells of the microenvironment locally or at distant sites. The uptake of circulating EVs and the responses by human monocytes in vitro may provide new insights into the underlying biology of the invasive and metastatic processes in cancer. Although a mixed population of vesicles is obtained with most isolation techniques, we predominantly isolated exosomes (small EVs) and microvesicles (medium EVs) from the SW480 colorectal cancer cell line (established from a primary adenocarcinoma of the colon) by sequential centrifugation and ultrafiltration, and plasma EVs were prepared from 22 patients with rectal adenoma polyps or invasive adenocarcinoma by size-exclusion chromatography. The EVs were thoroughly characterized. The uptake of SW480 EVs was analyzed, and small SW480 EVs were observed to be more potent than medium SW480 EVs in inducing monocyte secretion of cytokines. The plasma EVs were also internalized by monocytes; however, their cytokine-releasing potency was lower than that of the cell line-derived vesicles. The transcriptional changes in the monocytes highlighted differences between adenoma and adenocarcinoma patient EVs in their ability to regulate biological functions, whereas the most intriguing changes were found in monocytes receiving EVs from patients with metastatic compared with localized cancer.en_US
dc.publisherWiley Open Accessen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleUptake of circulating extracellular vesicles from rectal cancer patients and differential responses by human monocyte culturesen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.source.journalFEBS Open Bioen_US
dc.relation.projectAkershus universitetssykehus HF: 2018004en_US
dc.relation.projectHelse Sør-Øst RHF: 2019109en_US
dc.relation.projectHelse Sør-Øst RHF: 2014010en_US
dc.relation.projectHelse Sør-Øst RHF: 2016050en_US
dc.relation.projectHelse Sør-Øst RHF: 2018056en_US
dc.relation.projectHelse Sør-Øst RHF: 2017109en_US
dc.relation.projectAkershus universitetssykehus HF: 2017013en_US

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Navngivelse 4.0 Internasjonal
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