dc.contributor.author | Alfano, Niccolò | |
dc.contributor.author | Dayaram, Anisha | |
dc.contributor.author | Axtner, Jan | |
dc.contributor.author | Tsangaras, Kyriakos | |
dc.contributor.author | Kampmann, Marie-Louise | |
dc.contributor.author | Mohamed, Azlan | |
dc.contributor.author | Wong, Seth T. | |
dc.contributor.author | Gilbert, Marcus Thomas Pius | |
dc.contributor.author | Wilting, Andreas | |
dc.contributor.author | Greenwood, Alex D. | |
dc.date.accessioned | 2022-03-04T12:22:55Z | |
dc.date.available | 2022-03-04T12:22:55Z | |
dc.date.created | 2021-12-20T15:45:07Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Methods in Ecology and Evolution. 2021, 12 (10), 1941-1952. | en_US |
dc.identifier.issn | 2041-210X | |
dc.identifier.uri | https://hdl.handle.net/11250/2983159 | |
dc.description.abstract | Environmental DNA (eDNA) and invertebrate-derived DNA (iDNA) are used to survey biodiversity non-invasively to mitigate difficulties in obtaining wildlife samples, particularly in remote areas or for rare species. Recently, eDNA/iDNA were used to monitor known wildlife pathogens; however, most wildlife pathogens are unknown and often evolutionarily divergent.
To detect and identify known and novel mammalian viruses from eDNA/iDNA, we used a curated set of RNA oligonucleotides as viral baits in a hybridization capture system coupled with high-throughput sequencing.
We detected multiple known and novel mammalian RNA and DNA viruses from multiple viral families from both waterhole eDNA and leech-derived iDNA. Congruence was found between detected hosts and viruses identified in leeches and waterholes.
Our results demonstrate that eDNA/iDNA samples represent an effective non-invasive resource for studying wildlife viral diversity and for detecting novel potentially zoonotic viruses prior to their emergence. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Wiley | en_US |
dc.rights | Navngivelse-Ikkekommersiell 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/deed.no | * |
dc.title | Non-invasive surveys of mammalian viruses using environmental DNA | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 1941-1952 | en_US |
dc.source.volume | 12 | en_US |
dc.source.journal | Methods in Ecology and Evolution | en_US |
dc.source.issue | 10 | en_US |
dc.identifier.doi | 10.1111/2041-210X.13661 | |
dc.identifier.cristin | 1970660 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |