dc.contributor.author | Shanbhag, Siddharth | |
dc.contributor.author | Elsebahy, Ahmad Rashad | |
dc.contributor.author | Helgeland Nymark, Ellen | |
dc.contributor.author | Suliman, Salwa | |
dc.contributor.author | Davies, Catharina de Lange | |
dc.contributor.author | Stavropoulos, Andreas | |
dc.contributor.author | Bolstad, Anne Isine | |
dc.contributor.author | Mustafa, Kamal Babikeir Elnour | |
dc.date.accessioned | 2022-02-15T09:33:21Z | |
dc.date.available | 2022-02-15T09:33:21Z | |
dc.date.created | 2021-09-03T15:40:58Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Frontiers in Bioengineering and Biotechnology. 2021, 9, . | en_US |
dc.identifier.issn | 2296-4185 | |
dc.identifier.uri | https://hdl.handle.net/11250/2979010 | |
dc.description.abstract | Cell coculture strategies can promote angiogenesis within tissue engineering constructs. This study aimed to test the angiogenic potential of human umbilical vein endothelial cells (HUVEC) cocultured with gingiva-derived progenitor cells (GPC) as spheroids in a xeno-free environment. Human platelet lysate (HPL) was used as a cell culture supplement and as a hydrogel matrix (HPLG) for spheroid encapsulation. HUVEC and HUVEC + GPC (1:1 or 5:1) spheroids were encapsulated in various HPLG formulations. Angiogenesis was assessed via in vitro sprouting and in vivo chick chorioallantoic membrane (CAM) assays. HUVEC revealed characteristic in vitro sprouting in HPL/HPLG and this was significantly enhanced in cocultures with GPC (p < 0.05). A trend for greater sprouting was observed in 5:1 vs 1:1 HUVEC + GPC spheroids and in certain HPLG formulations (p > 0.05). Both HUVEC and HUVEC + GPC spheroids in HPLG revealed abundant and comparable neoangiogenesis in the CAM assay (p > 0.05). Spheroid coculture of HUVEC + GPC in HPLG represents a promising strategy to promote angiogenesis. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Frontiers Media | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Spheroid Coculture of Human Gingiva-Derived Progenitor Cells With Endothelial Cells in Modified Platelet Lysate Hydrogels | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.volume | 9 | en_US |
dc.source.journal | Frontiers in Bioengineering and Biotechnology | en_US |
dc.identifier.doi | 10.3389/fbioe.2021.739225 | |
dc.identifier.cristin | 1931229 | |
dc.source.articlenumber | 739225 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |