Single Cell Confocal Raman Spectroscopy of Human Osteoarthritic Chondrocytes: A Preliminary Study
Kumar, Rajesh; Singh, Gajendra Pratap; Grønhaug, Kirsten Marie; Afseth, Nils Kristian; Davies, Catharina De Lange; Drogset, Jon Olav; Lilledahl, Magnus Borstad
Journal article, Peer reviewed
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http://hdl.handle.net/11250/283842Utgivelsesdato
2015Metadata
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Originalversjon
International Journal of Molecular Sciences 2015, 16(5):9341-9353 10.3390/ijms16059341Sammendrag
A great deal of effort has been focused on exploring the underlying molecular
mechanism of osteoarthritis (OA) especially at the cellular level. We report a confocal
Raman spectroscopic investigation on human osteoarthritic chondrocytes. The objective of
this investigation is to identify molecular features and the stage of OA based on the
spectral signatures corresponding to bio-molecular changes at the cellular level in
chondrocytes. In this study, we isolated chondrocytes from human osteoarthritic cartilage
and acquired Raman spectra from single cells. Major spectral differences between the
cells obtained from different International Cartilage Repair Society (ICRS) grades of
osteoarthritic cartilage were identified. During progression of OA, a decrease in protein
content and an increase in cell death were observed from the vibrational spectra. Principal
component analysis and subsequent cross-validation was able to associate osteoarthritic
chondrocytes to ICRS Grade I, II and III with specificity 100.0%, 98.1%, and 90.7%
respectively, while, sensitivity was 98.6%, 82.8%, and 97.5% respectively. The overall
predictive efficiency was 92.2%. Our pilot study encourages further use of Raman
spectroscopy as a noninvasive and label free technique for revealing molecular features
associated with osteoarthritic chondrocytes.
Keywords: Raman spectroscopy; osteoarthritis; chondrocytes; biomedical optical diagnosis