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dc.contributor.authorMargaryan, Ashot
dc.contributor.authorNoer, Christina Lehmkuhl
dc.contributor.authorRichter, Stine R.
dc.contributor.authorRestrup, Marlene Elise
dc.contributor.authorBülow-Hansen, Julie Lee
dc.contributor.authorLeerhøi, Frederik
dc.contributor.authorLangkjær, Emilia Marie Rolander
dc.contributor.authorGopalakrishnan, Shyam
dc.contributor.authorCarøe, Christian
dc.contributor.authorGilbert, Marcus Thomas Pius
dc.contributor.authorBohmann, Kristine
dc.date.accessioned2021-12-15T10:35:59Z
dc.date.available2021-12-15T10:35:59Z
dc.date.created2021-12-07T13:25:18Z
dc.date.issued2021
dc.identifier.citationEnvironmental DNA. 2021, 3 (2), 472-480.en_US
dc.identifier.issn2637-4943
dc.identifier.urihttps://hdl.handle.net/11250/2834379
dc.description.abstractBiodiversity monitoring projects using environmental DNA techniques are becoming increasingly widespread. However, these techniques depend heavily on the quality and richness of the available DNA reference database against which the DNA sequences are queried. To create a comprehensive DNA sequence database for future DNA-based biodiversity assessments in Denmark, a national DNA reference database, DNAmark, was established, which contains organellar and/or nuclear reference data from vouchered museum species of plants, animals, and fungi from Denmark. Here, we present full or partial mitochondrial genomes of 182 Danish vertebrate species representing ca. 22% of vertebrate species observed in Denmark. Further, we demonstrate that storage conditions of the specimens accounted for ca. 50% of the total variation in mitochondrial DNA (mtDNA) preservation while the age of museum specimens had little effect: ca. 4%. In addition, we roughly estimate the cost of sequencing to be 25 EUR per specimen for obtaining sufficient amounts of DNA reads (ca. 200-fold coverage) for reliable mitogenome assemblies while also obtaining low coverage genomic data. The large number of mitogenomes of Danish vertebrate species represents the initial groundwork for DNA-based biodiversity assessments of vertebrates in Denmark and paves the way for practitioners to freely choose mitochondrial DNA markers.en_US
dc.language.isoengen_US
dc.publisherJohn Wiley & Sons Ltd.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleMitochondrial genomes of Danish vertebrate species generated for the national DNA reference database, DNAmarken_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.pagenumber472-480en_US
dc.source.volume3en_US
dc.source.journalEnvironmental DNAen_US
dc.source.issue2en_US
dc.identifier.doi10.1002/edn3.138
dc.identifier.cristin1965597
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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