dc.contributor.author | Quiles Jimenez, Ana Maria Teresa | |
dc.contributor.author | Gregersen, Ida | |
dc.contributor.author | Sousa, Mirta | |
dc.contributor.author | Abbas, Azhar | |
dc.contributor.author | Kong, Xiang Yi | |
dc.contributor.author | Alseth, Ingrun | |
dc.contributor.author | Holm, Sverre | |
dc.contributor.author | Dahl, Tuva Børresdatter | |
dc.contributor.author | Skagen, Karolina Ryeng | |
dc.contributor.author | Skjelland, Mona | |
dc.contributor.author | Aukrust, Pål | |
dc.contributor.author | Bjørås, Magnar | |
dc.contributor.author | Halvorsen, Bente | |
dc.date.accessioned | 2021-03-18T11:41:27Z | |
dc.date.available | 2021-03-18T11:41:27Z | |
dc.date.created | 2020-10-13T12:32:04Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Biochemical and Biophysical Research Communications - BBRC. 2020, 1-7. | en_US |
dc.identifier.issn | 0006-291X | |
dc.identifier.uri | https://hdl.handle.net/11250/2734172 | |
dc.description.abstract | Background
More than 170 post-transcriptional RNA modifications regulate the localization, processing and function of cellular RNAs, and aberrant RNA modifications have been linked to a range of human diseases. The RNA modification landscape in atherosclerosis, the main underlying cause of cardiovascular diseases, is still largely unknown.
Methods
We used mass spectrometry to analyse a selection of RNA-modifying enzymes and the N6-methyladenosine (m6A) in carotid atherosclerotic lesion samples representing early and advanced stages of atherosclerosis as compared to non-atherosclerotic arteries from healthy controls.
Findings
(i) the detection of different levels of several enzymes involved in methylations occurring in rRNA and mRNA; (ii) these findings included changes in the levels of methyltransferases (‘writers’), binding proteins (‘readers’) and demethylases (‘erasers’) during atherosclerosis as compared to non-atherosclerotic control arteries, with generally the most prominent differences in samples from early atherosclerotic lesions; and (iii) these changes were accompanied by a marked downregulation of m6A in rRNA, the most abundant and well-studied modification in mRNA with a wide range of effects on cell biology.
Interpretation
We show for the first time that RNA-modifying enzymes and the well-studied RNA modification m6A are differentially regulated in atherosclerotic lesions, which potentially could help creating new prognostic and treatment strategies. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no | * |
dc.title | N6-methyladenosine in RNA of atherosclerotic plaques: An epitranscriptomic signature of human carotid atherosclerosis | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 1-7 | en_US |
dc.source.journal | Biochemical and Biophysical Research Communications - BBRC | en_US |
dc.identifier.doi | 10.1016/j.bbrc.2020.09.057 | |
dc.identifier.cristin | 1839170 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |