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dc.contributor.authorSubbannayya, Yashwanth
dc.contributor.authorHaug, Markus
dc.contributor.authorPinto, Sneha
dc.contributor.authorMohanty, Varshasnata
dc.contributor.authorMeås, Hany Zakaria
dc.contributor.authorFlo, Trude Helen
dc.contributor.authorPrasad, T. S. Keshava
dc.contributor.authorKandasamy, Richard Kumaran
dc.description.abstractCD4+ T cells (T helper cells) are cytokine-producing adaptive immune cells that activate or regulate the responses of various immune cells. The activation and functional status of CD4+ T cells is important for adequate responses to pathogen infections but has also been associated with auto-immune disorders and survival in several cancers. In the current study, we carried out a label-free high-resolution FTMS-based proteomic profiling of resting and T cell receptor-activated (72 h) primary human CD4+ T cells from peripheral blood of healthy donors as well as SUP-T1 cells. We identified 5237 proteins, of which significant alterations in the levels of 1119 proteins were observed between resting and activated CD4+ T cells. In addition to identifying several known T-cell activation-related processes altered expression of several stimulatory/inhibitory immune checkpoint markers between resting and activated CD4+ T cells were observed. Network analysis further revealed several known and novel regulatory hubs of CD4+ T cell activation, including IFNG, IRF1, FOXP3, AURKA, and RIOK2. Comparison of primary CD4+ T cell proteomic profiles with human lymphoblastic cell lines revealed a substantial overlap, while comparison with mouse CD+ T cell data suggested interspecies proteomic differences. The current dataset will serve as a valuable resource to the scientific community to compare and analyze the CD4+ proteome.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleThe Proteomic Landscape of Resting and Activated CD4+ T Cells Reveal Insights into Cell Differentiation and Functionen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.subject.nsiVDP::Medisinske fag: 700en_US
dc.subject.nsiVDP::Midical sciences: 700en_US
dc.source.journalInternational Journal of Molecular Sciencesen_US
dc.relation.projectSamarbeidsorganet mellom Helse Midt-Norge og NTNU: 90176000en_US
dc.relation.projectNorges forskningsråd: 223255en_US
dc.description.localcodeThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citeden_US

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