Show simple item record

dc.contributor.authorLegøy, Thomas Aga
dc.contributor.authorVethe, Heidrun
dc.contributor.authorAbadpour, Shadab
dc.contributor.authorStrand, Berit Løkensgard
dc.contributor.authorScholz, Hanne
dc.contributor.authorPaulo, Joao A.
dc.contributor.authorRæder, Helge
dc.contributor.authorGhila, Luiza
dc.contributor.authorChera, Simona
dc.date.accessioned2020-11-13T14:16:23Z
dc.date.available2020-11-13T14:16:23Z
dc.date.created2020-09-28T16:17:32Z
dc.date.issued2020
dc.identifier.citationScientific Reports. 2020, 10, .en_US
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/11250/2687869
dc.description.abstractCell replacement therapies hold great therapeutic potential. Nevertheless, our knowledge of the mechanisms governing the developmental processes is limited, impeding the quality of differentiation protocols. Generating insulin-expressing cells in vitro is no exception, with the guided series of differentiation events producing heterogeneous cell populations that display mixed pancreatic islet phenotypes and immaturity. The achievement of terminal differentiation ultimately requires the in vivo transplantation of, usually, encapsulated cells. Here we show the impact of cell confinement on the pancreatic islet signature during the guided differentiation of alginate encapsulated human induced pluripotent stem cells (hiPSCs). Our results show that encapsulation improves differentiation by significantly reshaping the proteome landscape of the cells towards an islet-like signature. Pathway analysis is suggestive of integrins transducing the encapsulation effect into intracellular signalling cascades promoting differentiation. These analyses provide a molecular framework for understanding the confinement effects on hiPSCs differentiation while confirming its importance for this process.en_US
dc.language.isoengen_US
dc.publisherNature Researchen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleEncapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signallingen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.volume10en_US
dc.source.journalScientific Reportsen_US
dc.source.issue10en_US
dc.identifier.doi10.1038/s41598-019-57305-x
dc.identifier.cristin1834455
dc.description.localcodeOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.source.articlenumber414en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal