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dc.contributor.authorFotakis, Anna K.
dc.contributor.authorDenham, Sean Dexter
dc.contributor.authorMackie, Meaghan
dc.contributor.authorOrbegozo, Miren Iraeta
dc.contributor.authorMylopotamitaki, Dorothea
dc.contributor.authorGopalakrishnan, Shyam
dc.contributor.authorSicheritz-Pontén, Thomas
dc.contributor.authorOlsen, Jesper V.
dc.contributor.authorCappellini, Enrico
dc.contributor.authorZhang, Guojie
dc.contributor.authorChristophersen, Axel
dc.contributor.authorGilbert, Thomas
dc.contributor.authorVågene, Åshild J
dc.identifier.citationPhilosophical Transactions of the Royal Society of London. Biological Sciences. 2020, 375 (1812), .en_US
dc.description.abstractMineralized dental plaque (calculus) has proven to be an excellent source of ancient biomolecules. Here we present a Mycobacterium leprae genome (6.6-fold), the causative agent of leprosy, recovered via shotgun sequencing of sixteenth-century human dental calculus from an individual from Trondheim, Norway. When phylogenetically placed, this genome falls in branch 3I among the diversity of other contemporary ancient strains from Northern Europe. Moreover, ancient mycobacterial peptides were retrieved via mass spectrometry-based proteomics, further validating the presence of the pathogen. Mycobacterium leprae can readily be detected in the oral cavity and associated mucosal membranes, which likely contributed to it being incorporated into this individual’s dental calculus. This individual showed some possible, but not definitive, evidence of skeletal lesions associated with early-stage leprosy. This study is the first known example of successful multi-omics retrieval of M. leprae from archaeological dental calculus. Furthermore, we offer new insights into dental calculus as an alternative sample source to bones or teeth for detecting and molecularly characterizing M. leprae in individuals from the archaeological record. This article is part of the theme issue ‘Insights into health and disease from ancient biomolecules’.en_US
dc.publisherThe Royal Societyen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleMulti-omic detection of Mycobacterium leprae in archaeological human dental calculusen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.source.journalPhilosophical Transactions of the Royal Society of London. Biological Sciencesen_US
dc.description.localcode© 2020 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License, which permits unrestricted use, provided the original author and source are credited.en_US

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