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Cost-Effective Live Cell Density Determination of Liquid Cultured Microorganisms

Kutschera, Alexander; Lamb, Jacob Joseph
Peer reviewed, Journal article
Accepted version
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URI
https://hdl.handle.net/11250/2674383
Date
2017
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  • Publikasjoner fra CRIStin - NTNU [20888]
Original version
Current Microbiology. 2017, 75 (2), 231-236.   10.1007/s00284-017-1370-3
Abstract
Live monitoring of microorganisms growth in liquid medium is a desired parameter for many research fields. A wildly used approach for determining microbial liquid growth quantification is based on light scattering as the result of the physical interaction of light with microbial cells. These measurements are generally achieved using costly table-top instruments; however, a live, reliable, and straight forward instrument constructed using parts that are inexpensive may provide opportunities for many researchers. Here, such an instrument has been constructed and tested. It consists of modular test tube holding chambers, each with a low power monochromatic light-emitting diode, and a monolithic photodiode. A microcontroller connects to all modular chambers to control the diodes, and send the live data to either an LCD screen, or a computer. This work demonstrate that this modular instrument can determine precise cell concentrations for the bacteria Escherichia coli and Pseudomonas syringae pv. tomato DC3000, as well as Saccharomyces cerevisiae yeast.
Publisher
Springer Verlag
Journal
Current Microbiology

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