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dc.contributor.authorLe, Simone Balzer
dc.contributor.authorOnsager, Ingerid
dc.contributor.authorLorentzen, Jon Andreas
dc.contributor.authorLale, Rahmi
dc.description.abstractBacterial 50 untranslated regions of mRNA (UTR) involve in a complex regulation of gene expression; however, the exact sequence features contributing to gene regulation are not yet fully understood. In this study, we report the design of a novel 50 UTR, dual UTR, utilising the transcriptional and translational characteristics of 50 UTRs in a single expression cassette. The dual UTR consists of two 50 UTRs, each separately leading to either increase in transcription or translation of the reporter, that are separated by a spacer region, enabling de novo translation initiation. We rationally create dual UTRs with a wide range of expression profiles and demonstrate the functionality of the novel design concept in Escherichia coli and Pseudomonas putida using different promoter systems and coding sequences. Overall, we demonstrate the application potential of dual UTR design concept in various synthetic biology applications ranging from fine-tuning of gene expression to maximisation of protein production.en_US
dc.publisherOxford University Pressen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleDual UTR-A novel 5′ untranslated region design for synthetic biology applicationsen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.source.journalSynthetic Biologyen_US
dc.description.localcode© The Author(s) 2020. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal