dc.contributor.author | Krzeminski, Pawel | |
dc.contributor.author | Feys, Edward | |
dc.contributor.author | Anglès d'Auriac, Marc | |
dc.contributor.author | Wennberg, Aina Charlotte | |
dc.contributor.author | Umar, Muhammad | |
dc.contributor.author | Schwermer, Carsten Ulrich | |
dc.contributor.author | Uhl, Wolfgang | |
dc.date.accessioned | 2020-06-29T08:35:14Z | |
dc.date.available | 2020-06-29T08:35:14Z | |
dc.date.created | 2019-12-03T22:17:06Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Journal of Membrane Science. 2020, 598 . | en_US |
dc.identifier.issn | 0376-7388 | |
dc.identifier.uri | https://hdl.handle.net/11250/2659798 | |
dc.description.abstract | The removal of cell free DNA (plasmids) carrying antibiotic resistance genes (ARGs) was investigated at bench-scale using ultrafiltration (UF), nanofiltration (NF) and reverse osmosis (RO) membranes commonly applied in water reuse applications. The removal of the plasmid spiked to ultrapure water was determined using a direct qPCR method. More than 99% plasmid removal was achieved by membranes with 1 kDa molecular weight cut off (MWCO). Membranes with lower MWCO showed complete removal under the specific experimental conditions, reaching a maximum log reduction value above 6.6. The concentrate from membrane filtration was further subjected to UV-LED irradiation at 265 nm. The required fluence for 1 log damage was 73 mJ/cm2 for the 267 target bp segment and 23 mJ/cm2 for the 601 target bp segment, respectively. With these two DNA segments, the inactivation rate per segment length was higher for the larger segment, in accordance with a higher pyrimidine and TT content, compared with the smaller fragment. Target DNA was not detectable anymore when using 100 and 300 mJ/cm2 for the 601 and 267 bp segments respectively. The results indicate that membrane filtration, combined with UV-LED treatment of the concentrate, can be an effective measure to remove and inactivate ARGs from water to prevent their release to the environment. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Combined membrane filtration and 265 nm UV irradiation for effective removal of cell free antibiotic resistance genes from feed water and concentrate | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 11 | en_US |
dc.source.volume | 598 | en_US |
dc.source.journal | Journal of Membrane Science | en_US |
dc.identifier.doi | 10.1016/j.memsci.2019.117676 | |
dc.identifier.cristin | 1756310 | |
dc.relation.project | Norges forskningsråd: 160016 | en_US |
dc.description.localcode | This is an open access article distributed under the terms of the Creative Commons CC-BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |