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dc.contributor.authorParelius Jonasova, Eleonora
dc.contributor.authorBjørkøy, Astrid
dc.contributor.authorStokke, Bjørn Torger
dc.identifier.citationJournal of Biomedical Optics. 2016, 21 (12), .en_US
dc.description.abstractOptical aberrations due to refractive index mismatches occur in various types of microscopy due to refractive differences between the sample and the immersion fluid or within the sample. We study the effects of lateral refractive index differences by fluorescence confocal laser scanning microscopy due to glass or polydimethylsiloxane cuboids and glass cylinders immersed in aqueous fluorescent solution, thereby mimicking realistic imaging situations in the proximity of these materials. The reduction in fluorescence intensity near the embedded objects was found to depend on the geometry and the refractive index difference between the object and the surrounding solution. The observed fluorescence intensity gradients do not reflect the fluorophore concentration in the solution. It is suggested to apply a Gaussian fit or smoothing to the observed fluorescence intensity gradient and use this as a basis to recover the fluorophore concentration in the proximity of the refractive index step change. The method requires that the reference and sample objects have the same geometry and refractive index. The best results were obtained when the sample objects were also used for reference since small differences such as uneven surfaces will result in a different extent of aberration.en_US
dc.publisherSociety of Photo-optical Instrumentation Engineersen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleRecovering fluorophore concentration profiles from confocal images near lateral refractive index step changesen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.source.journalJournal of Biomedical Opticsen_US
dc.description.localcode© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE) [DOI: 10.1117/1.JBO.21.12.126014] CC-BY licenseen_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal