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dc.contributor.authorIslam, Mohammad Mirazul
dc.contributor.authorSharifi, Roholah
dc.contributor.authorMamodaly, Shamina
dc.contributor.authorIslam, Rakibul
dc.contributor.authorNahra, Daniel
dc.contributor.authorAbusamra, Dina B.
dc.contributor.authorHui, Pui Chuen
dc.contributor.authorAdibnia, Yashar
dc.contributor.authorGoulamaly, Mehdi
dc.contributor.authorPaschalis, Eleftherios I.
dc.contributor.authorCruzat, Andrea
dc.contributor.authorKong, Jing
dc.contributor.authorNilsson, Per
dc.contributor.authorArgüeso, Pablo
dc.contributor.authorMollnes, Tom Eirik
dc.contributor.authorChodosh, James
dc.contributor.authorDohlman, Claes H.
dc.contributor.authorGonzalez-Andrades, Miguel
dc.identifier.citationActa Biomaterialia. 2019, 1-15.nb_NO
dc.description.abstractTo address the shortcomings associated with corneal transplants, substantial efforts have been focused on developing new modalities such as xenotransplantion. Xenogeneic corneas are anatomically and biomechanically similar to the human cornea, yet their applications require prior decellularization to remove the antigenic components to avoid rejection. In the context of bringing decellularized corneas into clinical use, sterilization is a crucial step that determines the success of the transplantation. Well-standardized sterilization methods, such as gamma irradiation (GI), have been applied to decellularized porcine corneas (DPC) to avoid graft-associated infections in human recipients. However, little is known about the effect of GI on decellularized corneal xenografts. Here, we evaluated the radiation effect on the ultrastructure, optical, mechanical and biological properties of DPC. Transmission electron microscopy revealed that gamma irradiated decellularized porcine cornea (G-DPC) preserved its structural integrity. Moreover, the radiation did not reduce the optical properties of the tissue. Neither DPC nor G-DPC led to further activation of complement system compared to native porcine cornea when exposed to plasma. Although, DPC were mechanically comparable to the native tissue, GI increased the mechanical strength, tissue hydrophobicity and resistance to enzymatic degradation. Despite these changes, human corneal epithelial, stromal, endothelial and hybrid neuroblastoma cells grew and differentiated on DPC and G-DPC. Thus, GI may achieve effective tissue sterilization without affecting critical properties that are essential for corneal transplant survival.nb_NO
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.titleEffects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenograftsnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.source.journalActa Biomaterialianb_NO
dc.description.localcode© 2019. This is the authors’ accepted and refereed manuscript to the article. Locked until 5.7.2021 due to copyright restrictions. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
cristin.unitnameNorges teknisk-naturvitenskapelige universitet

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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal