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dc.contributor.authorNarverud, Ingunn
dc.contributor.authorChristensen, Jacob J.
dc.contributor.authorBakke, Siril Skaret
dc.contributor.authorUlven, Stine Marie
dc.contributor.authorRundblad, Amanda
dc.contributor.authorAukrust, Pål
dc.contributor.authorEspevik, Terje
dc.contributor.authorBogsrud, Martin Prøven
dc.contributor.authorRetterstøl, Kjetil
dc.contributor.authorUeland, Thor
dc.contributor.authorHalvorsen, Bente
dc.contributor.authorHolven, Kirsten Bjørklund
dc.identifier.citationJournal of Internal Medicine. 2019, 1-12.nb_NO
dc.description.abstractBackground Innate and adaptive immune responses are pivotal in atherosclerosis, but their association with early‐stage atherosclerosis in humans is incompletely understood. In this regard, untreated children with familial hypercholesterolaemia may serve as a human model to investigate the effect of elevated low‐density lipoprotein (LDL)‐cholesterol. Objectives We aimed to study the immunological and inflammatory pathways involved in early atherosclerosis by examining mRNA molecules in peripheral blood mononuclear cells (PBMCs) from children with FH. Methods We analysed the level of 587 immune‐related mRNA molecules using state‐of‐the‐art Nanostring technology in PBMCs from children with (n = 30) and without (n = 21) FH, and from FH children before and after statin therapy (n = 10). Results 176 genes (30%) were differentially expressed between the FH and healthy children at P < 0.05. Compared to healthy children, the dysregulated pathways in FH children included the following: T cells (18/19); B cells (5/6); tumour necrosis factor super family (TNFSF) (6/8); cell growth, proliferation and differentiation (5/7); interleukins (5/9); toll‐like receptors (2/5); apoptosis (3/7) and antigen presentation (1/7), where the ratio denotes higher expressed genes to total number of genes. Statin therapy reversed expression of thirteen of these mRNAs in FH children. Conclusion FH children display higher PBMC expression of immune‐related genes mapped to several pathways, including T and B cells, and TNFSF than healthy children. Our results suggest that LDL‐C plays an important role in modulating expression of different immune‐related genes, and novel data on the involvement of these pathways in the early atherosclerosis may represent future therapeutic targets for prevention of atherosclerotic progression.nb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleProfiling of immune-related gene expression in children with familial hypercholesterolaemianb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.source.journalJournal of Internal Medicinenb_NO
dc.description.localcodeª 2019 The Authors. Journal of Internal Medicine published by John Wiley & Sons Ltd on behalf of Association for Publication 1 This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly citednb_NO
cristin.unitnameInstitutt for klinisk og molekylær medisin

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal