Generally applicable procedure for in situ formation of fluorescent protein-gold nanoconstructs
dc.contributor.author | Volden, Sondre | |
dc.contributor.author | Lystvet, Sina Maria | |
dc.contributor.author | Halskau jr, Øyvind | |
dc.contributor.author | Glomm, Wilhelm | |
dc.date.accessioned | 2019-10-16T15:34:22Z | |
dc.date.available | 2019-10-16T15:34:22Z | |
dc.date.created | 2012-12-04T13:50:20Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | RSC Advances. 2012, 2 (31), 11704-11711. | nb_NO |
dc.identifier.issn | 2046-2069 | |
dc.identifier.uri | http://hdl.handle.net/11250/2622653 | |
dc.description.abstract | Small noble metal nanoclusters can be formed in situ by direct reduction and stabilization of a metal precursor by biomolecules such as proteins. Considering the diversity in amino acid composition of proteins, and hence their reductive ability, a general method for synthesis of gold nanoclusters using proteins is presented here. A range of proteins (bovine serum albumin, fibrinogen, α-lactalbumin, lysozyme, cytochrome c, myoglobin, β-lactoglobulin and α-chymotrypsin) have been studied, based on size, isoelectric point, flexibility and 3-dimensional structure. Results show protein-gold nanoconstructs with complex protein-specific photophysical properties. The effect on the 3-dimensional conformation of the proteins upon formation of gold nanoclusters and/or nanoparticles within the protein structure is also shown to be highly protein-dependent. A general mechanism for the formation of protein-gold nanoconstructs is proposed, based on charge density matching, yielding a high local concentration of the metal precursor on the protein structure which in turn can nucleate, grow and be stabilized by amino acid residues in the protein. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Royal Society of Chemistry | nb_NO |
dc.title | Generally applicable procedure for in situ formation of fluorescent protein-gold nanoconstructs | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.subject.nsi | VDP::Nanoteknologi: 630 | nb_NO |
dc.subject.nsi | VDP::Nanotechnology: 630 | nb_NO |
dc.source.pagenumber | 11704-11711 | nb_NO |
dc.source.volume | 2 | nb_NO |
dc.source.journal | RSC Advances | nb_NO |
dc.source.issue | 31 | nb_NO |
dc.identifier.doi | 10.1039/c2ra21931j | |
dc.identifier.cristin | 967868 | |
dc.description.localcode | This article will not be available due to copyright restrictions (c) 2012 by RCA | nb_NO |
cristin.unitcode | 194,66,30,0 | |
cristin.unitname | Institutt for kjemisk prosessteknologi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 |