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Generally applicable procedure for in situ formation of fluorescent protein-gold nanoconstructs

Volden, Sondre; Lystvet, Sina Maria; Halskau jr, Øyvind; Glomm, Wilhelm
Journal article, Peer reviewed
Published version
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Volden.pdf (Locked)
URI
http://hdl.handle.net/11250/2622653
Date
2012
Metadata
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  • Institutt for kjemisk prosessteknologi [1206]
  • Publikasjoner fra CRIStin - NTNU [19694]
Original version
RSC Advances. 2012, 2 (31), 11704-11711.   10.1039/c2ra21931j
Abstract
Small noble metal nanoclusters can be formed in situ by direct reduction and stabilization of a metal precursor by biomolecules such as proteins. Considering the diversity in amino acid composition of proteins, and hence their reductive ability, a general method for synthesis of gold nanoclusters using proteins is presented here. A range of proteins (bovine serum albumin, fibrinogen, α-lactalbumin, lysozyme, cytochrome c, myoglobin, β-lactoglobulin and α-chymotrypsin) have been studied, based on size, isoelectric point, flexibility and 3-dimensional structure. Results show protein-gold nanoconstructs with complex protein-specific photophysical properties. The effect on the 3-dimensional conformation of the proteins upon formation of gold nanoclusters and/or nanoparticles within the protein structure is also shown to be highly protein-dependent. A general mechanism for the formation of protein-gold nanoconstructs is proposed, based on charge density matching, yielding a high local concentration of the metal precursor on the protein structure which in turn can nucleate, grow and be stabilized by amino acid residues in the protein.
Publisher
Royal Society of Chemistry
Journal
RSC Advances

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