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dc.contributor.authorRobertson, Adam Brian
dc.contributor.authorDahl, John Arne
dc.contributor.authorVågbø, Cathrine Broberg
dc.contributor.authorTripathi, P
dc.contributor.authorKrokan, Hans Einar
dc.contributor.authorKlungland, Arne
dc.identifier.citationNucleic Acids Research. 2011, 39 (8), .nb_NO
dc.description.abstractRecently, 5-hydroxymethylcytosine (5hmC) was identified in mammalian genomic DNA. The biological role of this modification remains unclear; however, identifying the genomic location of this modified base will assist in elucidating its function. We describe a method for the rapid and inexpensive identification of genomic regions containing 5hmC. This method involves the selective glucosylation of 5hmC residues by the b-glucosyltransferase from T4 bacteriophage creating b-glucosyl-5-hydroxymethylcytosine (b-glu-5hmC). The b-glu-5hmC modification provides a target that can be efficiently and selectively pulled down by J-binding protein 1 coupled to magnetic beads. DNA that is precipitated is suitable for analysis by quantitative PCR, microarray or sequencing. Furthermore, we demonstrate that the J-binding protein 1 pull down assay identifies 5hmC at the promoters of developmentally regulated genes in human embryonic stem cells. The method described here will allow for a greater understanding of the temporal and spatial effects that 5hmC may have on epigenetic regulation at the single gene level.nb_NO
dc.publisherOxford University Pressnb_NO
dc.relation.uriA novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA
dc.rightsNavngivelse-Ikkekommersiell 4.0 Internasjonal*
dc.titleA novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNAnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.source.journalNucleic Acids Researchnb_NO
dc.description.localcodeThe Author(s) 2011. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.nb_NO
cristin.unitnameInstitutt for klinisk og molekylær medisin

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Navngivelse-Ikkekommersiell 4.0 Internasjonal
Med mindre annet er angitt, så er denne innførselen lisensiert som Navngivelse-Ikkekommersiell 4.0 Internasjonal