dc.contributor.author | Robertson, Adam Brian | |
dc.contributor.author | Dahl, John Arne | |
dc.contributor.author | Vågbø, Cathrine Broberg | |
dc.contributor.author | Tripathi, P | |
dc.contributor.author | Krokan, Hans Einar | |
dc.contributor.author | Klungland, Arne | |
dc.date.accessioned | 2019-10-11T11:09:04Z | |
dc.date.available | 2019-10-11T11:09:04Z | |
dc.date.created | 2011-08-29T13:30:04Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Nucleic Acids Research. 2011, 39 (8), . | nb_NO |
dc.identifier.issn | 0305-1048 | |
dc.identifier.uri | http://hdl.handle.net/11250/2621582 | |
dc.description.abstract | Recently, 5-hydroxymethylcytosine (5hmC) was identified in mammalian genomic DNA. The biological role of this modification remains unclear; however, identifying the genomic location of this modified base will assist in elucidating its function. We describe a method for the rapid and inexpensive identification of genomic regions containing 5hmC. This method involves the selective glucosylation of 5hmC residues by the b-glucosyltransferase from T4 bacteriophage creating b-glucosyl-5-hydroxymethylcytosine (b-glu-5hmC). The b-glu-5hmC modification provides a target that can be efficiently and selectively pulled down by J-binding protein 1 coupled to magnetic beads. DNA that is precipitated is suitable for analysis by quantitative PCR, microarray or sequencing. Furthermore, we demonstrate that the J-binding protein 1 pull down assay identifies 5hmC at the promoters of developmentally regulated genes in human embryonic stem cells. The method described here will allow for a greater understanding of the temporal and spatial effects that 5hmC may have on epigenetic regulation at the single gene level. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Oxford University Press | nb_NO |
dc.relation.uri | A novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA | |
dc.rights | Navngivelse-Ikkekommersiell 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/deed.no | * |
dc.title | A novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.pagenumber | 10 | nb_NO |
dc.source.volume | 39 | nb_NO |
dc.source.journal | Nucleic Acids Research | nb_NO |
dc.source.issue | 8 | nb_NO |
dc.identifier.doi | 10.1093/nar/gkr051 | |
dc.identifier.cristin | 835402 | |
dc.description.localcode | The Author(s) 2011. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. | nb_NO |
cristin.unitcode | 194,65,15,0 | |
cristin.unitname | Institutt for klinisk og molekylær medisin | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |