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dc.contributor.authorVan den Born, Erwin
dc.contributor.authorVågbø, Cathrine Broberg
dc.contributor.authorSonge-Møller, Lene
dc.contributor.authorLeihne, VIbeke
dc.contributor.authorLien, Guro Flor
dc.contributor.authorLeszczynska, G
dc.contributor.authorMalkiewicz, A
dc.contributor.authorKrokan, Hans Einar
dc.contributor.authorKirpekar, F
dc.contributor.authorKlungland, Arne
dc.contributor.authorFalnes, Pål
dc.date.accessioned2019-10-10T11:10:02Z
dc.date.available2019-10-10T11:10:02Z
dc.date.created2011-09-13T12:41:51Z
dc.date.issued2011
dc.identifier.citationNature Communications. 2011, 2 .nb_NO
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/11250/2621379
dc.description.abstractMammals have nine different homologues (ALKBH1–9) of the Escherichia coli DNA repair demethylase AlkB. ALKBH2 is a genuine DNA repair enzyme, but the in vivo function of the other ALKBH proteins has remained elusive. It was recently shown that ALKBH8 contains an additional transfer RNA (tRNA) methyltransferase domain, which generates the wobble nucleoside 5-methoxycarbonylmethyluridine (mcm5U) from its precursor 5-carboxymethyluridine (cm5U). In this study, we report that (R)- and (S)-5-methoxycarbonylhydroxymethyluridine (mchm5U), hydroxylated forms of mcm5U, are present in mammalian , and , respectively, representing the first example of a diastereomeric pair of modified RNA nucleosides. Through in vitro and in vivo studies, we show that both diastereomers of mchm5U are generated from mcm5U, and that the AlkB domain of ALKBH8 specifically hydroxylates mcm5U into (S)-mchm5U in . These findings expand the function of the ALKBH oxygenases beyond nucleic acid repair and increase the current knowledge on mammalian wobble uridine modifications and their biogenesis.nb_NO
dc.language.isoengnb_NO
dc.publisherNature Researchnb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNAnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber7nb_NO
dc.source.volume2nb_NO
dc.source.journalNature Communicationsnb_NO
dc.identifier.doi10.1038/ncomms1173
dc.identifier.cristin838894
dc.description.localcodeCreative Commons Attribution Licensenb_NO
cristin.unitcode194,65,15,0
cristin.unitnameInstitutt for klinisk og molekylær medisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2


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