dc.contributor.advisor | Sletmoen, Marit | |
dc.contributor.advisor | Beatson, Richard | |
dc.contributor.author | Monshaugen, Ida | |
dc.date.accessioned | 2019-09-11T09:47:55Z | |
dc.date.created | 2018-05-28 | |
dc.date.issued | 2018 | |
dc.identifier | ntnudaim:16196 | |
dc.identifier.uri | http://hdl.handle.net/11250/2615533 | |
dc.description.abstract | The experimental data regarding interaction studies and energy landscape presented in this master thesis were obtained using a dual-beam Optical Tweezers. The main objective of this study was to investigate the binding of Siglec-9 to MUC1-ST and to PAA-ST to determine the differences in affinity. Results obtained indicated that MUC1-ST interacts with Siglec-9 with a rupture force of 7.2-22.2 pN for loading rates ranging from 53-174 pN. Estimates of parameters characteristic for the energy landscape resulted in k_off between 0.92-2.2 s-1, and x_β from 0.35-1.40 nm. For PAA-ST interacting with Siglec-9 the rupture force was 10.2-34.2 pN for a loading rate ranging from 50-189 pN, k_off between 1.0-1.9 s-1, and x_β from 0.33-1.30 nm. MUC1-ST was found to interact with Siglec-9 at a higher frequency compared to PAA-ST. | en |
dc.language | eng | |
dc.publisher | NTNU | |
dc.subject | Bioteknologi (5 årig), Molekylærbiologi | en |
dc.title | Elucidating binding capacities of different sialic acid-carrying polymer substrates with Siglec-9 by Optical Tweezers - Studies of the importance of mucin glycosylation for cancer progression | en |
dc.type | Master thesis | en |
dc.source.pagenumber | 87 | |
dc.contributor.department | Norges teknisk-naturvitenskapelige universitet, Fakultet for naturvitenskap,Institutt for bioteknologi og matvitenskap | nb_NO |
dc.date.embargoenddate | 10000-01-01 | |