dc.contributor.author | Pischke, Søren | |
dc.contributor.author | Hestenes, Siv Merete | |
dc.contributor.author | Johansen, Harald Thidemann | |
dc.contributor.author | Fure, Hilde | |
dc.contributor.author | Bugge, Jan F | |
dc.contributor.author | Espinoza, Andreas Westenvik | |
dc.contributor.author | Skulstad, Helge | |
dc.contributor.author | Edvardsen, Thor | |
dc.contributor.author | Fosse, Erik | |
dc.contributor.author | Mollnes, Tom Eirik | |
dc.contributor.author | Halvorsen, Per Steinar | |
dc.contributor.author | Nielsen, Erik Waage | |
dc.date.accessioned | 2019-09-10T05:56:47Z | |
dc.date.available | 2019-09-10T05:56:47Z | |
dc.date.created | 2019-09-07T12:39:56Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | PLOS ONE. 2019, 14:e0218624 (6), 1-16. | nb_NO |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/11250/2614351 | |
dc.description.abstract | Introduction
Right ventricular (RV) myocardial dysfunction is a common feature in septic shock. It can worsen outcome, but the etiology is poorly understood. Pulmonary artery hypertension (PAH) plays a part in the pathogenesis of the right heart dysfunction in sepsis but its importance is unknown. In pigs, PAH in sepsis is substantial and the translational value of porcine sepsis models therefore questioned. We hypothesized that porcine sepsis causes a myocardial inflammatory response which leads to myocardial dysfunction independent of PAH.
Materials and methods
Sepsis was induced by Escherichia coli-infusion in 10 pigs resulting in PAH and increased right ventricular pressure (RVP). The same degree of RVP was achieved by external pulmonary artery banding (PAB) in a consecutive series of 6 animals.
Results
Sepsis, but not PAB, led to increase in endothelial damage marker PAI-1 and cytokines TNF and IL-6 (all p<0.05) in plasma. In myocardium, TNF and IL-6 were significantly elevated in sepsis, TNF in both ventricles and IL-6 mostly in RV, while IL-1β, IL-18 and C5a were significantly higher in RV compared to LV after PAB (all p<0.05). Myocardial mRNA levels of IL-1β, IL-6, IL-18, IP-10, E-selectin and PAI-1 were significantly elevated in RV and LV during sepsis compared to PAB, while Caspase-1 was decreased in septic compared to PAB animals (all p<0.05). Cathepsin L activity was increased in RV by PAB, while sepsis inhibited this response. Escherichia coli-induced sepsis caused myocardial inflammation independent of PAH.
Conclusion
Prominent PAH should therefore not exclude porcine sepsis models to further our understanding of human sepsis. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Public Library of Science | nb_NO |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.pagenumber | 1-16 | nb_NO |
dc.source.volume | 14:e0218624 | nb_NO |
dc.source.journal | PLOS ONE | nb_NO |
dc.source.issue | 6 | nb_NO |
dc.identifier.doi | 10.1371/journal.pone.0218624 | |
dc.identifier.cristin | 1722520 | |
dc.description.localcode | © 2019 Pischke et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited | nb_NO |
cristin.unitcode | 194,65,15,0 | |
cristin.unitname | Institutt for klinisk og molekylær medisin | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |