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dc.contributor.authorUrbancsok, Janos
dc.contributor.authorBones, Atle M.
dc.contributor.authorKissen, Ralph
dc.date.accessioned2018-07-10T07:15:58Z
dc.date.available2018-07-10T07:15:58Z
dc.date.created2018-06-16T10:43:12Z
dc.date.issued2018
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11250/2504892
dc.description.abstractUpon tissue damage the plant secondary metabolites glucosinolates can generate various hydrolysis products, including isothiocyanates (ITCs). Their role in plant defence against insects and pest and their potential health benefits have been well documented, but our knowledge regarding the endogenous molecular mechanisms of their effect in plants is limited. Here we investigated the effect of allyl-isothiocyanate (AITC) on Arabidopsis thaliana mutants impaired in homeostasis of the low-molecular weight thiol glutathione. We show that glutathione is important for the AITC-induced physiological responses, since mutants deficient in glutathione biosynthesis displayed a lower biomass and higher root growth inhibition than WT seedlings. These mutants were also more susceptible than WT to another ITC, sulforaphane. Sulforaphane was however more potent in inhibiting root growth than AITC. Combining AITC with the glutathione biosynthesis inhibitor L-buthionine-sulfoximine (BSO) led to an even stronger phenotype than observed for the single treatments. Furthermore, transgenic plants expressing the redox-sensitive fluorescent biomarker roGFP2 indicated more oxidative conditions during AITC treatment. Taken together, we provide genetic evidence that glutathione plays an important role in AITC-induced growth inhibition, although further studies need to be conducted to reveal the underlying mechanisms.nb_NO
dc.language.isoengnb_NO
dc.publisherNature Publishing Groupnb_NO
dc.relation.urihttps://rdcu.be/14zZ
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleArabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanatenb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.volume8nb_NO
dc.source.journalScientific Reportsnb_NO
dc.identifier.doi10.1038/s41598-018-28099-1
dc.identifier.cristin1591649
dc.relation.projectNorges forskningsråd: 214329nb_NO
dc.description.localcode© The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License.nb_NO
cristin.unitcode194,66,10,0
cristin.unitnameInstitutt for biologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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