dc.contributor.author | Stafsnes, Marit Hallvardsdotter | |
dc.contributor.author | Røst, Lisa Marie | |
dc.contributor.author | Bruheim, Per | |
dc.date.accessioned | 2018-04-05T13:28:11Z | |
dc.date.available | 2018-04-05T13:28:11Z | |
dc.date.created | 2018-04-03T12:15:23Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Journal of chromatography. B. 2018, 1083 278-283. | nb_NO |
dc.identifier.issn | 1570-0232 | |
dc.identifier.uri | http://hdl.handle.net/11250/2492906 | |
dc.description.abstract | The phosphometabolome is comprised of all phosphorylated metabolites including the major metabolite classes sugar phosphates and nucleoside phosphates. Phosphometabolites are invaluable in any cell as a part of primary- and energy- metabolism, and as building blocks in the biosynthesis of macromolecules. Here, we report quantitative profiling of the phosphometabolome by applying capillary ion chromatography-tandem mass spectrometry (capIC-MS/MS), ensuring improved chromatographic separation, robustness and quantitative precision. Baseline separation was achieved for six out of eight tested hexose phosphates. Quantitative precision and reproducibility was improved by introducing a fully uniformly (U) 13C–labeled biological extract and applying an isotope dilution (ID) correction strategy. A 13C–labeled biological extract does in principle contain internal standards (IS) for all metabolites, but low abundant metabolites pose a challenge, and solutions to this are discussed. The extreme reproducibility and reliability of this capIC-MS/MS method was demonstrated by running the instrumentation continuously for ten days. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Elsevier | nb_NO |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no | * |
dc.title | Improved phosphometabolome profiling applying isotope dilution strategy and capillary ion chromatography-tandem mass spectrometry | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | acceptedVersion | nb_NO |
dc.source.pagenumber | 278-283 | nb_NO |
dc.source.volume | 1083 | nb_NO |
dc.source.journal | Journal of chromatography. B | nb_NO |
dc.identifier.doi | 10.1016/j.jchromb.2018.02.004 | |
dc.identifier.cristin | 1576785 | |
dc.relation.project | Norges forskningsråd: 237165 | nb_NO |
dc.description.localcode | © 2018. This is the authors’ accepted and refereed manuscript to the article. Locked until 6.2.2020 due to copyright restrictions. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | nb_NO |
cristin.unitcode | 194,66,15,0 | |
cristin.unitname | Institutt for bioteknologi og matvitenskap | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |