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dc.contributor.authorGago-Fuentes, Raquel
dc.contributor.authorXing, Mengtan
dc.contributor.authorSæterstad, Siri
dc.contributor.authorSarno, Antonio
dc.contributor.authorDewan, Alisa Elinsdatter
dc.contributor.authorBeck, Carole
dc.contributor.authorBradamante, Stefano
dc.contributor.authorBjørås, Magnar
dc.contributor.authorOksenych, Valentyn
dc.description.abstractDNA repair consists of several cellular pathways which recognize and repair damaged DNA. The classical nonhomologous DNA end-joining (NHEJ) pathway repairs double-strand breaks in DNA. It is required for maturation of both B and T lymphocytes by supporting V(D)J recombination as well as B-cell differentiation during class switch recombination (CSR). Inactivation of NHEJ factors Ku70, Ku80, XRCC4, DNA ligase 4, DNA-PKcs, and Artemis impairs V(D)J recombination and blocks lymphocyte development. Paralogue of XRCC4 and XLF (PAXX) is an accessory NHEJ factor that has a significant impact on the repair of DNA lesions induced by ionizing radiation in human, murine, and chicken cells. However, the role of PAXX during development is poorly understood. To determine the physiological role of PAXX, we deleted part of the Paxx promoter and the first two exons in mice. Further, we compared Paxx-knockout mice with wild-type (WT) and NHEJ-deficient controls including Ku80- and Dna-pkcs-null and severe combined immunodeficiency mice. Surprisingly, Paxx-deficient mice were not distinguishable from the WT littermates; they were the same weight and size, fertility status, had normal spleen, thymus, and bone marrow. Paxx-deficient mice had the same number of chromosomal and chromatid breaks as WT mice. Moreover, Paxx-deficient primary B lymphocytes had the same level of CSR as lymphocytes isolated from WT mice. We concluded that PAXX is dispensable for normal mouse development.nb_NO
dc.publisherWiley Open Accessnb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleNormal development of mice lacking PAXX, the paralogue of XRCC4 and XLFnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.source.journalFEBS Open Bionb_NO
dc.description.localcode© 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License.nb_NO
cristin.unitnameInstitutt for klinisk og molekylær medisin

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