dc.contributor.author | Andrade, Warrison A | |
dc.contributor.author | Agarwal, Sarika | |
dc.contributor.author | Mo, Shunyan | |
dc.contributor.author | Shaffer, Scott A | |
dc.contributor.author | Dillard, Joseph P | |
dc.contributor.author | Schmidt, Tobias | |
dc.contributor.author | Hornung, Veit | |
dc.contributor.author | Fitzgerald, Katherine A. | |
dc.contributor.author | Kurt-Jones, Evelyn A. | |
dc.contributor.author | Golenbock, Douglas T | |
dc.date.accessioned | 2018-01-03T08:29:55Z | |
dc.date.available | 2018-01-03T08:29:55Z | |
dc.date.created | 2016-12-12T14:32:09Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Cell reports. 2016, 15 (11), 2438-2448. | nb_NO |
dc.identifier.issn | 2211-1247 | |
dc.identifier.uri | http://hdl.handle.net/11250/2474202 | |
dc.description.abstract | The innate immune system is the first line of defense against Neisseria gonorrhoeae (GC). Exposure of cells to GC lipooligosaccharides induces a strong immune response, leading to type I interferon (IFN) production via TLR4/MD-2. In addition to living freely in the extracellular space, GC can invade the cytoplasm to evade detection and elimination. Double-stranded DNA introduced into the cytosol binds and activates the enzyme cyclic-GMP-AMP synthase (cGAS), which produces 2′3′-cGAMP and triggers STING/TBK-1/IRF3 activation, resulting in type I IFN expression. Here, we reveal a cytosolic response to GC DNA that also contributes to type I IFN induction. We demonstrate that complete IFN-β induction by live GC depends on both cGAS and TLR4. Type I IFN is detrimental to the host, and dysregulation of iron homeostasis genes may explain lower bacteria survival in cGAS−/− and TLR4−/− cells. Collectively, these observations reveal cooperation between TLRs and cGAS in immunity to GC infection. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Elsevier | nb_NO |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no | * |
dc.title | Type I Interferon Induction by Neisseria gonorrhoeae: Dual Requirement of Cyclic GMP-AMP Synthase and Toll-like Receptor 4 | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.pagenumber | 2438-2448 | nb_NO |
dc.source.volume | 15 | nb_NO |
dc.source.journal | Cell reports | nb_NO |
dc.source.issue | 11 | nb_NO |
dc.identifier.doi | 10.1016/j.celrep.2016.05.030 | |
dc.identifier.cristin | 1411595 | |
dc.relation.project | Norges forskningsråd: 223255 | nb_NO |
dc.description.localcode | © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/) | nb_NO |
cristin.unitcode | 194,65,15,0 | |
cristin.unitname | Institutt for klinisk og molekylær medisin | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |