Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette
| dc.contributor.author | Zwick, Friederike | |
| dc.contributor.author | Lale, Rahmi | |
| dc.contributor.author | Valla, Svein | |
| dc.date.accessioned | 2017-11-29T11:36:27Z | |
| dc.date.available | 2017-11-29T11:36:27Z | |
| dc.date.created | 2012-11-22T12:45:29Z | |
| dc.date.issued | 2012 | |
| dc.identifier.citation | Microbial Cell Factories. 2012, 11:133. | nb_NO |
| dc.identifier.issn | 1475-2859 | |
| dc.identifier.uri | http://hdl.handle.net/11250/2468515 | |
| dc.description.abstract | The XylS/Pm expression system has been used to produce recombinant proteins at industrial levels in Escherichia coli. Activation of transcription from the Pm promoter takes place in the presence of benzoic acid or derivatives of it. Previous mutagenesis studies resulted in identification of several variants of the expression control elements xylS (X), Pm (P) and the 5'-untranslated region (U) that individually gave rise to strongly stimulated expression. The goal of this study was to test if combination of such stimulatory mutations in the same expression vectors would lead to further increase of expression levels. | nb_NO |
| dc.language.iso | eng | nb_NO |
| dc.publisher | BioMed Central | nb_NO |
| dc.rights | Navngivelse 4.0 Internasjonal | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
| dc.title | Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette | nb_NO |
| dc.type | Journal article | nb_NO |
| dc.type | Peer reviewed | nb_NO |
| dc.description.version | publishedVersion | nb_NO |
| dc.source.volume | 11 | nb_NO |
| dc.source.journal | Microbial Cell Factories | nb_NO |
| dc.source.issue | 133 | nb_NO |
| dc.identifier.doi | 10.1186/1475-2859-11-133 | |
| dc.identifier.cristin | 964186 | |
| dc.relation.project | Norges forskningsråd: 192123 | nb_NO |
| dc.description.localcode | © Zwick et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | nb_NO |
| cristin.unitcode | 194,66,15,0 | |
| cristin.unitname | Institutt for bioteknologi og matvitenskap | |
| cristin.ispublished | true | |
| cristin.fulltext | original | |
| cristin.qualitycode | 1 |
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