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dc.contributor.authorPérez-Sánchez, Jaume
dc.contributor.authorTerova, Genciana
dc.contributor.authorSimó-Mirabet, Paula
dc.contributor.authorRimoldi, Simona
dc.contributor.authorFolkedal, Ole
dc.contributor.authorCalduch-Giner, Josep Alvar
dc.contributor.authorOlsen, Rolf Erik
dc.contributor.authorSitjà-Bobadilla, Ariadna
dc.date.accessioned2017-11-28T09:54:03Z
dc.date.available2017-11-28T09:54:03Z
dc.date.created2017-06-14T15:51:58Z
dc.date.issued2017
dc.identifier.citationFrontiers in Physiology. 2017, 8:34, 1-18.nb_NO
dc.identifier.issn1664-042X
dc.identifier.urihttp://hdl.handle.net/11250/2468205
dc.description.abstractThe skin mucus of gilthead sea bream was mapped by one-dimensional gel electrophoresis followed by liquid chromatography coupled to high resolution mass spectrometry using a quadrupole time-of-flight mass analyzer. More than 2,000 proteins were identified with a protein score filter of 30. The identified proteins were represented in 418 canonical pathways of the Ingenuity Pathway software. After filtering by canonical pathway overlapping, the retained proteins were clustered in three groups. The mitochondrial cluster contained 59 proteins related to oxidative phosphorylation and mitochondrial dysfunction. The second cluster contained 79 proteins related to antigen presentation and protein ubiquitination pathways. The third cluster contained 257 proteins where proteins related to protein synthesis, cellular assembly, and epithelial integrity were over-represented. The latter group also included acute phase response signaling. In parallel, two-dimensional gel electrophoresis methodology identified six proteins spots of different protein abundance when comparing unstressed fish with chronically stressed fish in an experimental model that mimicked daily farming activities. The major changes were associated with a higher abundance of cytokeratin 8 in the skin mucus proteome of stressed fish, which was confirmed by immunoblotting. Thus, the increased abundance of markers of skin epithelial turnover results in a promising indicator of chronic stress in fish.nb_NO
dc.language.isoengnb_NO
dc.publisherFrontiers Medianb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleSkin mucus of gilthead sea bream (sparus aurata l.). protein mapping and regulation in chronically stressed fishnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber1-18nb_NO
dc.source.volume8:34nb_NO
dc.source.journalFrontiers in Physiologynb_NO
dc.identifier.doi10.3389/fphys.2017.00034
dc.identifier.cristin1476148
dc.relation.projectEC/FP7/262336nb_NO
dc.description.localcode© 2017 Pérez-Sánchez, Terova, Simó-Mirabet, Rimoldi, Folkedal, Calduch-Giner, Olsen and Sitjà-Bobadilla. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.nb_NO
cristin.unitcode194,66,10,0
cristin.unitnameInstitutt for biologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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