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dc.contributor.authorIbstedt, Sebastian
dc.contributor.authorStenberg, Simon
dc.contributor.authorBages, Sara
dc.contributor.authorGjuvsland, Arne Bjørke
dc.contributor.authorSalinas, Francisco
dc.contributor.authorKourtchenko, Olga
dc.contributor.authorSamy, Jeevan K.A.
dc.contributor.authorBlomberg, Anders
dc.contributor.authorOmholt, Stig William
dc.contributor.authorLiti, Gianni
dc.contributor.authorBeltran, Gemma
dc.contributor.authorWarringer, Jonas
dc.date.accessioned2017-10-24T07:52:42Z
dc.date.available2017-10-24T07:52:42Z
dc.date.created2015-01-22T13:54:50Z
dc.date.issued2015
dc.identifier.citationMolecular biology and evolution. 2015, 32 (1), 153-161.nb_NO
dc.identifier.issn0737-4038
dc.identifier.urihttp://hdl.handle.net/11250/2461600
dc.description.abstractExposing natural selection driving phenotypic and genotypic adaptive differentiation is an extraordinary challenge. Given that an organism’s life stages are exposed to the same environmental variations, we reasoned that fitness components, such as the lag, rate, and efficiency of growth, directly reflecting performance in these life stages, should often be selected in concert. We therefore conjectured that correlations between fitness components over natural isolates, in a particular environmental context, would constitute a robust signal of recent selection. Critically, this test for selection requires fitness components to be determined by different genetic loci. To explore our conjecture, we exhaustively evaluated the lag, rate, and efficiency of asexual population growth of natural isolates of the model yeast Saccharomyces cerevisiae in a large variety of nitrogen-limited environments. Overall, fitness components were well correlated under nitrogen restriction. Yeast isolates were further crossed in all pairwise combinations and coinheritance of each fitness component and genetic markers were traced. Trait variations tended to map to quantitative trait loci (QTL) that were private to a single fitness component. We further traced QTLs down to single-nucleotide resolution and uncovered loss-of-function mutations in RIM15, PUT4, DAL1, and DAL4 as the genetic basis for nitrogen source use variations. Effects of SNPs were unique for a single fitness component, strongly arguing against pleiotropy between lag, rate, and efficiency of reproduction under nitrogen restriction. The strong correlations between life stage performances that cannot be explained by pleiotropy compellingly support adaptive differentiation of yeast nitrogen source use and suggest a generic approach for detecting selection.nb_NO
dc.language.isoengnb_NO
dc.publisherOxford University Pressnb_NO
dc.titleConcerted Evolution of Life Stage Performances Signals Recent Selection on Yeast Nitrogen Usenb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionacceptedVersionnb_NO
dc.source.pagenumber153-161nb_NO
dc.source.volume32nb_NO
dc.source.journalMolecular biology and evolutionnb_NO
dc.source.issue1nb_NO
dc.identifier.doi10.1093/molbev/msu285
dc.identifier.cristin1204903
dc.relation.projectNotur/NorStore: NN4653Knb_NO
dc.relation.projectNorges forskningsråd: 222364nb_NO
dc.description.localcodeThis is a pre-copyedited, author-produced version of an article accepted for publication in Molecular Biology and Evolution following peer review. The version of record is available online at: https://academic.oup.com/mbe/article-lookup/doi/10.1093/molbev/msu285nb_NO
cristin.unitcode194,66,15,0
cristin.unitnameInstitutt for bioteknologi og matvitenskap
cristin.ispublishedtrue
cristin.fulltextpostprint
cristin.qualitycode2


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