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dc.contributor.authorBeckwith, Kai Muller
dc.contributor.authorSikorski, Pawel
dc.date.accessioned2017-10-09T08:15:48Z
dc.date.available2017-10-09T08:15:48Z
dc.date.created2014-01-02T11:25:17Z
dc.date.issued2013
dc.identifier.issn1758-5082
dc.identifier.urihttp://hdl.handle.net/11250/2459099
dc.description.abstractLive cell arrays are an emerging tool that expand traditional 2D in vitro cell culture, increasing experimental precision and throughput. A patterned cell system was developed by combining the cell-repellent properties of polyvinyl alcohol hydrogels with the cell adhesive properties of self-assembled films of dopamine (polydopamine). It was shown that polydopamine could be patterned onto spin-cast polyvinyl alcohol hydrogels by microcontact printing, which in turn effectively patterned the growth of several cell types (HeLa, human embryonic kidney, human umbilical vein endothelial cells (HUVEC) and prostate cancer). The cells could be patterned in geometries down to single-cell confinement, and it was demonstrated that cell patterns could be maintained for at least 3 weeks. Furthermore, polydopamine could be used to modify poly(vinyl alcohol) in situ using a cell-compatible deposition buffer (1 mg mL−1 dopamine in 25 mM tris with a physiological salt balance). The treatment switched the PVA hydrogel from cell repellent to cell adhesive. Finally, by combining microcontact printing and in situ deposition of polydopamine, patterned co-cultures of the same cell type (HeLa/HeLa) and dissimilar cell types (HeLa/HUVEC) were realized through simple chemistry and could be studied over time. The combination of polyvinyl alcohol and polydopamine was shown to be an attractive route to versatile, patterned cell culture experiments with minimal infrastructure requirements and low complexity.nb_NO
dc.language.isoengnb_NO
dc.publisherIOP Publishingnb_NO
dc.titlePatterned cell arrays and patterned co-cultures on polydopamine-modified poly(vinyl alcohol) hydrogelsnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionacceptedVersionnb_NO
dc.source.volume5nb_NO
dc.source.journalBiofabricationnb_NO
dc.source.issue4nb_NO
dc.identifier.doi10.1088/1758-5082/5/4/045009
dc.identifier.cristin1082185
dc.description.localcode© 2013 IOP Publishing. This is the authors' accepted and refereed manuscript to the article.nb_NO
cristin.unitcode194,66,20,0
cristin.unitnameInstitutt for fysikk
cristin.ispublishedtrue
cristin.fulltextpostprint
cristin.qualitycode1


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