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Mass spectrometry- based metabolite analyses in Streptomyces coelicolor

Klubicka, Lidija
Master thesis
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URI
http://hdl.handle.net/11250/245753
Date
2011
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  • Institutt for bioteknologi og matvitenskap [899]
Abstract
A set of mutants with inactivated genes encoding different parts of the butanolide system in Streptomyces coelicolor are cultivated in fermenters. We want to identify and characterize the signaling molecules, gamma- butyrolactones and their role in context to antibiotic production. Extracellular concentrations of the gamma- butyrolactones are determined by LC-MS. Also, intracellular metabolites in a mutant with an inactivated gene responsible for a part of the nitrogen metabolism in S.coelicolor are analysed using GC-MS.
Publisher
Institutt for bioteknologi

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