Mass spectrometry- based metabolite analyses in Streptomyces coelicolor
Master thesis
Permanent lenke
http://hdl.handle.net/11250/245753Utgivelsesdato
2011Metadata
Vis full innførselSamlinger
Sammendrag
A set of mutants with inactivated genes encoding different parts of the butanolide system in Streptomyces coelicolor are cultivated in fermenters. We want to identify and characterize the signaling molecules, gamma- butyrolactones and their role in context to antibiotic production. Extracellular concentrations of the gamma- butyrolactones are determined by LC-MS. Also, intracellular metabolites in a mutant with an inactivated gene responsible for a part of the nitrogen metabolism in S.coelicolor are analysed using GC-MS.