Browsing Institutt for bioingeniørfag by Author "Thommesen, Liv"
Now showing items 1-20 of 21
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A high-throughput drug combination screen of targeted small molecule inhibitors in cancer cell lines
Flobak, Åsmund; Niederdorfer, Barbara; To, Vu; Thommesen, Liv; Klinkenberg, Geir; Lægreid, Astrid (Journal article; Peer reviewed, 2019)While there is a high interest in drug combinations in cancer therapy, openly accessible datasets for drug combination responses are sparse. Here we present a dataset comprising 171 pairwise combinations of 19 individual ... -
Automatisert ELISA metode for påvisning av IAA
Dørum, Therese; Andersen, Jenny Lund (Bachelor thesis, 2023)Bakgrunn: Enhet for immunologi har automatisert flere ELISA-tester, men fortsatt er det fem tester som utføres manuelt. Prosjektets hensikt er derfor å undersøke om en automatisering av en ELISA-test for insulin autoantistoff ... -
Characterisation of Colorectal Cancer Cell Lines through Proteomic Profiling of Their Extracellular Vesicles
Heck, Kathleen; Lindholm, Håvard Takle; Niederdorfer, Barbara; Tsirvouli, Eirini; Kuiper, Martin; Flobak, Åsmund; Lægreid, Astrid Renner; Thommesen, Liv (Journal article; Peer reviewed, 2023) -
Characterization of Mam301 in Fission yeast
Eidsmo, Nina Winje (Bachelor thesis, 2022)Background: Magnesium is an essential ion that is a cofactor for many important proteins and enzymes in the body. It also functions as a counterion for negative ions and thus helps stabilize many important molecules. ... -
Community assessment to advance computational prediction of cancer drug combinations in a pharmacogenomic screen
Kuiper, Martin; Lægreid, Astrid; Flobak, Åsmund; Thommesen, Liv; Team, Dream (Journal article; Peer reviewed, 2019)The effectiveness of most cancer targeted therapies is short-lived. Tumors often developresistance that might be overcome with drug combinations. However, the number of possiblecombinations is vast, necessitating data-driven ... -
Digital opplæring i urinmikroskopi
Hjelleset, Terese; Thomassen, Madelen Kristine (Bachelor thesis, 2020)Det er utfordrende å få tak i ferske nok uriner som inneholder tilstrekkelige variasjoner av normale og patologiske funn til opplæring i urinmikroskopi. Det har derfor lenge vært av interesse for både bioingeniørutdanningene ... -
Etablering av Real-Time PCR for deteksjon av Cutibacterium acnes
Kupen, Marita; Fredheim, Katrine Weilin (Bachelor thesis, 2021)Cutibacterium acnes er en gram positiv, anaerob stavbakterie som inngår i hudens normalflora. Bakterien kan være årsak til postoperative infeksjoner, da bakterien fester seg til proteser og kirurgisk utstyr. Per i dag ... -
Gastrin activates autophagy and increases migration and survival of gastric adenocarcinoma cells
Rao, Shalini; Solum, Guri; Niederdorfer, Barbara; Nørsett, Kristin; Bjørkøy, Geir; Thommesen, Liv (Journal article; Peer reviewed, 2017)Background: The peptide hormone gastrin exerts a growth-promoting effect in both normal and malignant gastrointestinal tissue. Gastrin mediates its effect via the cholecystokinin 2 receptor (CCKBR/CCK2R). Although a ... -
Gene regulation knowledge commons: community action takes care of DNA binding transcription factors
Tripathi, Sushil; Vercruysse, Steven; Chawla, Konika; Christie, Karen R.; Blake, Judith A; Huntley, Rachael P.; Orchard, Sandra; Hermjakob, Henning; Thommesen, Liv; Lægreid, Astrid; Kuiper, Martin (Journal article; Peer reviewed, 2016)A large gap remains between the amount of knowledge in scientific literature and the fraction that gets curated into standardized databases, despite many curation initiatives. Yet the availability of comprehensive knowledge ... -
High-throughput screening reveals higher synergistic effect of MEK inhibitor combinations in colon cancer spheroids
Folkesson, Evelina; Niederdorfer, Barbara; To, Vu; Thommesen, Liv; Klinkenberg, Geir; Lægreid, Astrid; Flobak, Åsmund (Peer reviewed; Journal article, 2020)Drug combinations have been proposed to combat drug resistance, but putative treatments are challenged by low bench-to-bed translational efficiency. To explore the effect of cell culture format and readout methods on ... -
Holdbarhetsstudie - kjølig oppbevaring av prøver til analyse av PT-INR
Matberg, Karianne; Martinsen, Stine Karoline (Bachelor thesis, 2020)I dette forsøket ble det utført en holdbarhetsstudie hvor det ble undersøkt om blodprøver oppbevart kjølig (5°C) kunne gi et signifikant endret analysesvar i forhold tilsvarende prøve oppbevart i romtemperatur. Dette ble ... -
NR4A2 is regulated by Gastrin and involved in gastrin-induced migration and invasion of gastric adenocarcinoma cells
Misund, Kristine; Selvik, Linn-Karina M.; Rao, Shalini; Nørsett, Kristin Gabestad; Bakke, Ingunn; Sandvik, Arne; Lægreid, Astrid; Bruland, Torunn; Prestvik, Wenche S; Thommesen, Liv (Peer reviewed; Journal article, 2013)The peptide hormone gastrin is known to play a role in differentiation, growth and apoptosis of cells in the gastric mucosa. In this study we demonstrate that gastrin induces Nuclear Receptor 4A2 (NR4A2) expression in the ... -
NR4A2 is regulated by Gastrin and involved in gastrin-induced migration and invasion of gastric adenocarcinoma cells
Misund, Kristine; Selvik, Linn-Karina M.; Rao, Shalini; Nørsett, Kristin Gabestad; Bakke, Ingunn; Sandvik, Arne; Lægreid, Astrid; Bruland, Torunn; Prestvik, Wenche S; Thommesen, Liv (Peer reviewed; Journal article, 2013)The peptide hormone gastrin is known to play a role in differentiation, growth and apoptosis of cells in the gastric mucosa. In this study we demonstrate that gastrin induces Nuclear Receptor 4A2 (NR4A2) expression in the ... -
Salt-inducible kinase 1 (SIK1) is induced by gastrin and inhibits migration of gastric adenocarcinoma cells
Selvik, Linn-Karina M.; Rao, Shalini; Steigedal, Tonje; Haltbakk, Ildri; Misund, Kristine; Bruland, Torunn; Prestvik, Wenche S; Lægreid, Astrid; Thommesen, Liv (Journal article; Peer reviewed, 2014-11-10)Salt-inducible kinase 1 (SIK1/Snf1lk) belongs to the AMP-activated protein kinase (AMPK) family of kinases, all of which play major roles in regulating metabolism and cell growth. Recent studies have shown that reduced ... -
Small RNA expression from viruses, bacteria and human miRNAs in colon cancer tissue and its association with microsatellite instability and tumor location
Mjelle, Robin; Sjursen, Wenche; Thommesen, Liv; Sætrom, Pål; Hofsli, Eva (Journal article; Peer reviewed, 2019)Background MicroRNAs (miRNA) and other small RNAs are frequently dysregulated in cancer and are promising biomarkers for colon cancer. Here we profile human, virus and bacteria small RNAs in normal and tumor tissue from ... -
The duration of gastrin treatment affects global gene expression and molecular responses involved in ER stress and anti-apoptosis
Selvik, Linn-Karina M.; Fjeldbo, Christina Sæten; Flatberg, Arnar; Steigedal, Tonje; Misund, Kristine; Anderssen, Endre; Doseth, Berit; Langaas, Mette; Tripathi, Sushil; Beisvag, Vidar; Lægreid, Astrid; Thommesen, Liv; Bruland, Torunn (Journal article; Peer reviewed, 2013)Background How cells decipher the duration of an external signal into different transcriptional outcomes is poorly understood. The hormone gastrin can promote a variety of cellular responses including proliferation, ... -
The gastrin and cholecystokinin receptors mediated signaling network: a scaffold for data analysis and new hypotheses on regulatory mechanisms
Tripathi, Sushil; Flobak, Åsmund; Chawla, Konika; Baudot, Anaïs; Bruland, Torunn; Thommesen, Liv; Kuiper, Martin Tremen R.; Lægreid, Astrid (Journal article; Peer reviewed, 2015) -
Transcriptional regulation by NR5A2 links differentiation and inflammation in the pancreas
Cobo, Isidoro; Martinelli, Paola; Flández, Marta; Bakiri, Latifa; Zhang, Mingfeng; Carrillo-de-Santa-Pau, Enrique; Jia, Jinping; Lobo, Víctor J. Sánchez-Arévalo; Megías, Diego; Felipe, Irene; del Pozo, Natalia; Millán, Irene; Thommesen, Liv; Bruland, Torunn; Olson, Sara H.; Smith, Jill; Schoonjans, Kristina; Bamlet, William R.; Petersen, Gloria M.; Malats, Núria; Amundadóttir, Laufey T.; Wagner, Erwin F.; Real, Francisco X. (Journal article; Peer reviewed, 2018)Chronic inflammation increases the risk of developing one of several types of cancer. Inflammatory responses are currently thought to be controlled by mechanisms that rely on transcriptional networks that are distinct from ... -
Utvikling og prevalidering av en sammenslått metode for kvantifisering av lamotrigin og mykofenolsyre i serum på UPLC-MSMS
Andersen, Per Ståle; Stenøien, Øyvind L.; Hofstad, Solvår (Bachelor thesis, 2021)Ved Avdeling for Klinisk Farmakologi (AKF) på St. Olavs Hospital blir de opprinnelige metodene for kvantifisering av lamotrigin og mykofenolsyre analysert daglig. Metodene benytter væskekromatografi og tandem massespektrometri ... -
Validering av kvantitativ analysemetode for kotinin i serum på UPLC-MS/MS
Bakkour, Ammar; Ullerud, Andreas; Vestrheim, Nikolai (Bachelor thesis, 2021)I denne oppgaven utførte vi en forenklet validering av en ny metode for kvantifisering av kotinin i serum på UPLC-MS/MS. Vi undersøkte også om metoden kunne benytte en standardrekke basert på 4% bovint serumalbumin fortynnet ...