dc.contributor.author | Nordli, Henriette R. | |
dc.contributor.author | Chinga-Carrasco, Gary | |
dc.contributor.author | Rokstad, Anne Mari | |
dc.contributor.author | Pukstad, Brita | |
dc.date.accessioned | 2016-10-03T14:45:52Z | |
dc.date.accessioned | 2016-10-10T14:34:19Z | |
dc.date.available | 2016-10-03T14:45:52Z | |
dc.date.available | 2016-10-10T14:34:19Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Carbohydrate Polymers 2016, 150:65-73 | nb_NO |
dc.identifier.issn | 1879-1344 | |
dc.identifier.uri | http://hdl.handle.net/11250/2414048 | |
dc.description.abstract | Wood cellulose nanofibrils (CNF) have been suggested as a potential wound healing material, but its utilization is limited by FDA requirements regarding endotoxin levels. In this study a method using sodium hydroxide followed by TEMPO mediated oxidation was developed to produce ultrapure cellulose nanofibrils, with an endotoxin level of 45 endotoxin units/g (EU/g) cellulose. Scanning transmission electron microscopy (S(T)EM) revealed a highly nanofibrillated structure (lateral width of 3.7 ± 1.3 nm).
Assessment of cytotoxicity and metabolic activity on Normal Human Dermal Fibroblasts and Human Epidermal Keratinocytes was done. CNF-dispersion of 50 μg/ml did not affect the cells. CNF-aerogels induced a reduction of metabolic activity by the fibroblasts and keratinocytes, but no significant cell death. Cytokine profiling revealed no induction of the 27 cytokines tested upon exposure to CNF. The moisture-holding capacity of aerogels was relatively high (∼7500%), compared to a commercially available wound dressing (∼2500%), indicating that the CNF material is promising as dressing material for management of wounds with a moderate to high amount of exudate. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Elsevier | nb_NO |
dc.subject | Ultrapure cellulose nanofibrils; Endotoxins; Keratinocytes; Fibroblasts; Cytotoxicity | nb_NO |
dc.title | Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.date.updated | 2016-10-03T14:45:52Z | |
dc.source.pagenumber | 65–73 | nb_NO |
dc.source.volume | 150 | nb_NO |
dc.source.journal | Carbohydrate Polymers | nb_NO |
dc.identifier.doi | 10.1016/j.carbpol.2016.04.094 | |
dc.identifier.cristin | 1363535 | |
dc.description.localcode | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | nb_NO |