Blar i Institutt for bioteknologi og matvitenskap på tidsskrift "PLoS ONE"
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A new and improved host-independent plasmid system for RK2-based conjugal transfer
(Journal article; Peer reviewed, 2014)Bacterial conjugation is a process that is mediated either by a direct cell-to-cell junction or by formation of a bridge between the cells. It is often used to transfer DNA constructs designed in Escherichia coli to recipient ... -
A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector
(Journal article, 2015)The fluorescence emission spectrum of photosynthetic microorganisms at liquid nitrogen temperature (77 K) provides important insights into the organization of the photosynthetic machinery of bacteria and eukaryotes, which ... -
Benzoic Acid-Inducible Gene Expression in Mycobacteria
(Journal article; Peer reviewed, 2015)Conditional expression is a powerful tool to investigate the role of bacterial genes. Here, we adapt the Pseudomonas putida-derived positively regulated XylS/Pm expression system to control inducible gene expression in ... -
Comparative analysis of weighted gene co-expression networks in human and mouse
(Journal article; Peer reviewed, 2017)The application of complex network modeling to analyze large co-expression data sets has gained traction during the last decade. In particular, the use of the weighted gene co-expression network analysis framework has ... -
Energy Landscape of Alginate-Epimerase Interactions Assessed by Optical Tweezers and Atomic Force Microscopy
(Journal article, 2015)Mannuronan C-5 epimerases are a family of enzymes that catalyze epimerization of alginates at the polymer level. This group of enzymes thus enables the tailor-making of various alginate residue sequences to attain various ... -
Functional Exploration of the Polysaccharide Lyase Family PL6
(Journal article; Peer reviewed, 2016)Alginate, the main cell-wall polysaccharide of brown algae, is composed of two residues: mannuronic acid (M-residues) and, its C5-epimer, guluronic acid (G-residues). Alginate lyases define a class of enzymes that cleave ... -
Functional Role of PilA in Iron Acquisition in the Cyanobacterium Synechocystis sp. PCC 6803
(Journal article; Peer reviewed, 2014)Cyanobacteria require large quantities of iron to maintain their photosynthetic machinery; however, in most environments iron is present in the form of insoluble iron oxides. Whether cyanobacteria can utilize these sources ... -
Genomics of Sponge-Associated Streptomyces spp. Closely Related to Streptomyces albus J1074: Insights into Marine Adaptation and Secondary Metabolite Biosynthesis Potential
(Journal article; Peer reviewed, 2014)A total of 74 actinomycete isolates were cultivated from two marine sponges, Geodia barretti and Phakellia ventilabrum collected at the same spot at the bottom of the Trondheim fjord (Norway). Phylogenetic analyses of ... -
Gradual Reduction in Sodium Content in Cooked Ham, with Corresponding Change in Sensorial Properties Measured by Sensory Evaluation and a Multimodal Machine Vision System
(Journal article; Peer reviewed, 2015)The European diet today generally contains too much sodium (Na+). A partial substitution of NaCl by KCl has shown to be a promising method for reducing sodium content. The aim of this work was to investigate the sensorial ... -
Interactions between the breast cancer-associated MUC1 mucins and C-type lectin characterized by optical tweezers
(Journal article; Peer reviewed, 2017)Carbohydrate–protein interactions govern many crucial processes in biological systems including cell recognition events. We have used the sensitive force probe optical tweezers to quantify the interactions occurring between ... -
Manganese acquisition is facilitated by PilA in the cyanobacterium Synechocystis sp. PCC 6803
(Journal article; Peer reviewed, 2017)Manganese is an essential element required by cyanobacteria, as it is an essential part of the oxygen-evolving center of photosystem II. In the presence of atmospheric oxygen, manganese is present as manganese oxides, which ... -
Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties
(Journal article; Peer reviewed, 2013-03-19)Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD+-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of ... -
Molecular adaptations to phosphorus deprivation and comparison with nitrogen deprivation responses in the diatom Phaeodactylum tricornutum
(Journal article; Peer reviewed, 2018)Phosphorus, an essential element for all living organisms, is a limiting nutrient in many regions of the ocean due to its fast recycling. Changes in phosphate (Pi) availability in aquatic systems affect diatom growth and ... -
Non-Invasive Analysis of Recombinant mRNA Stability in Escherichia coli by a Combination of Transcriptional Inducer Wash-Out and qRT-PCR
(Journal article; Peer reviewed, 2013)mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly ... -
Osteogenic differentiation of human mesenchymal stem cells in mineralized alginate matrices
(Journal article; Peer reviewed, 2015)Mineralized biomaterials are promising for use in bone tissue engineering. Culturing osteogenic cells in such materials will potentially generate biological bone grafts that may even further augment bone healing. Here, ... -
Structural Analysis of Glutaredoxin Domain of Mus musculus Thioredoxin Glutathione Reductase
(Journal article; Peer reviewed, 2012)Thioredoxin glutathione reductase (TGR) is a member of the mammalian thioredoxin reductase family that has a monothiol glutaredoxin (Grx) domain attached to the thioredoxin reductase module. Here, we report a structure ... -
The Design of Simple Bacterial Microarrays: Development towards Immobilizing Single Living Bacteria on Predefined Micro-Sized Spots on Patterned Surfaces
(Journal article; Peer reviewed, 2015)In this paper we demonstrate a procedure for preparing bacterial arrays that is fast, easy, and applicable in a standard molecular biology laboratory. Microcontact printing is used to deposit chemicals promoting bacterial ... -
The plastoquinone pool of Nannochloropsis oceanica is not completely reduced during bright light pulses
(Journal article; Peer reviewed, 2017)The lipid-producing model alga Nannochloropsis oceanica has a distinct photosynthetic machinery. This organism possesses chlorophyll a as its only chlorophyll species, and has a high ratio of PSI to PSII. This high ratio ... -
Transcriptional responses of Bacillus cereus towards challenges with the polysaccharide chitosan
(Journal article; Peer reviewed, 2011)The antibacterial activity of the polysaccharide chitosan towards different bacterial species has been extensively documented. The response mechanisms of bacteria exposed to this biopolymer and the exact molecular mechanism ... -
Unique photosynthetic electron transport tuning and excitation distribution in heterokont algae
(Journal article; Peer reviewed, 2019)Heterokont algae are significant contributors to marine primary productivity. These algae have a photosynthetic machinery that shares many common features with that of Viridiplantae (green algae and land plants). Here we ...