Browsing NTNU Open by Author "Tietze, Lisa"
Now showing items 1-8 of 8
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Characterization and industrial application of artificial promoter and 5' UTR sequences in Escherichia coli and Vibrio natriegens
Mangold, Antonia (Master thesis, 2021)Vibrio natriegens er en gramnegativ proteobakterie som i den siste tiden har fått mye oppmerksomhet på grunn av en rask doblingstid på under ti minutter. Den er fremvok- sende platform for syntetisk biologi, og det er ... -
Construction and characterisation of artificial promoters in Bacillus subtilis.
Lykke, Andreas (Master thesis, 2019)Bacillus subtilis en en gram-positiv modellorganisme. Den er mye brukt i både forskning og industri. B. subtilis regnes som en av de viktigste bakteriene for industrien, på grunn av effektivt uttrykk og sekresjon av ... -
Development and use of a novel gene expression engineering approach in synthetic biology applications
Tietze, Lisa (Doctoral theses at NTNU;2022:140, Doctoral thesis, 2022)Microorganisms have diverse biochemical capabilities that can be exploited to produce metabolites or proteins of interest, such as drugs, vitamins, or biofuels in industrial scale biomanufacturing processes. To enable ... -
Establishment and characterization of artificial promoter and 5′UTR sequences in Escherichia coli and Vibrio natriegens
Hoff, Maria Wilhelmsen (Master thesis, 2020)Forutsigbar og konsekvent regulering av protein produksjon er avgjørende når syntetiske genetiske kretser designes, og ved stor-skala industriell produksjon. For å oppnå dette er det nødvendig å identifisere eller designe ... -
Functional characterization of three Azotobacter chroococcum alginate-modifying enzymes related to the Azotobacter vinelandii AlgE mannuronan C-5-epimerase family
Gawin, Agnieszka Maria; Tietze, Lisa; Aarstad, Olav Andreas; Aachmann, Finn Lillelund; Brautaset, Trygve; Ertesvåg, Helga (Peer reviewed; Journal article, 2020)Bacterial alginate initially consists of 1-4-linked beta-D-mannuronic acid residues (M) which can be later epimerized to alpha-L-guluronic acid (G). The family of AlgE mannuronan C-5-epimerases from Azotobacter vinelandii ... -
Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens
Tietze, Lisa; Hoff, Maria Wilhelmsen; Lale, Rahmi (Peer reviewed; Journal article, 2022)Vibrio natriegens has recently gained attention as a novel fast-growing bacterium in synthetic biology applications. Currently, a limited set of genetic elements optimised for Escherichia coli are used in V. natriegens due ... -
Importance of the 5′ regulatory region to bacterial synthetic biology applications
Tietze, Lisa; Lale, Rahmi (Journal article; Peer reviewed, 2021) -
mCherry contains a fluorescent protein isoform that interferes with its reporter function
Fages-Lartaud, Maxime René; Tietze, Lisa; Elie, Florence; Lale, Rahmi; Hohmann-Marriott, Martin Frank (Peer reviewed; Journal article, 2022)Fluorescent proteins are essential reporters in cell and molecular biology. Here, we found that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform ...