• A targeted mass spectrometry immunoassay to quantify osteopontin in fresh-frozen breast tumors and adjacent normal breast tissues 

      Macur, Katarzyna; Hagen, Lars; Ciesielski, Thomasz M.; Konieczna, Lucyna; Skokowski, Jaroslaw; Jenssen, Bjørn Munro; Slupphaug, Geir; Baczek, Tomasz (Journal article; Peer reviewed, 2019)
      Osteopontin (OPN) is a multifunctional protein that can activate cell-signaling pathways and lead to cancer development and metastasis. Elevated OPN expression was reported in different cancer types, including breast tumors. ...
    • A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair 

      Brickner, Joshua R; Soll, Jennifer M; Lombardi, Patrick M; Vågbø, Cathrine Broberg; Mudge, Miranda C; Oyeniran, Clement; Rabe, Renana; Jackson, Jessica; Sullender, Meagan E; Blazosky, Elyse; Byrum, Andrea K; Zhao, Yu; Corbett, Mark A.; Gècz, Jozef; Field, Michael; Vindigni, Alessandro; Slupphaug, Geir; Wolberger, Cynthia; Mosammaparast, Nima (Journal article; Peer reviewed, 2017)
      DNA repair is essential to prevent the cytotoxic or mutagenic effects of various types of DNA lesions, which are sensed by distinct pathways to recruit repair factors specific to the damage type. Although biochemical ...
    • AID expression in B-cell lymphomas causes accumulation of genomic uracil and a distinct AID mutational signature 

      Pettersen, Henrik Sahlin; Galashevskaya, Anastasia; Doseth, Berit; Sousa, Mirta; Sarno, Antonio; Visnes, Torkild; Aas, Per Arne; Liabakk, Nina-Beate; Slupphaug, Geir; Sætrom, Pål; Kavli, Bodil Merete; Krokan, Hans Einar (Journal article; Peer reviewed, 2014)
      tThe most common mutations in cancer are C to T transitions, but their origin has remained elusive.Recently, mutational signatures of APOBEC-family cytosine deaminases were identified in many com-mon cancers, suggesting ...
    • An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells 

      Sousa, Mirta; Zub, Kamila Anna; Aas, Per Arne; Hanssen-Bauer, Audun; Demirovic, Aida; Sarno, Antonio; Tian, Erming; Liabakk, Nina-Beate; Slupphaug, Geir (Journal article; Peer reviewed, 2013)
      Alterations in checkpoint and DNA repair pathways may provide adaptive mechanisms contributing to acquired drug resistance. Here, we investigated the levels of proteins mediating DNA damage signaling and -repair in RPMI8226 ...
    • Antibody cross-linking and target elution protocols used for immunoprecipitation significantly modulate signal-to noise ratio in downstream 2D-PAGE analysis 

      Sousa, Mirta; Steen, Kristian Wollen; Hagen, Lars; Slupphaug, Geir (Journal article; Peer reviewed, 2011)
      Background Immunoprecipitation and subsequent 2D-PAGE/mass spectrometry are powerful tools to study post-translational protein modifications. Often disregarded in this workflow is the impact of the chemical cross-linker ...
    • Atrial Myocyte Function and Ca2+ Handling Is Associated with Inborn Aerobic Capacity 

      Johnsen, Anne Berit; Rolim, Natale Pinheiro Lage; Stølen, Tomas; Alves, Marcia; Sousa, Mirta; Slupphaug, Geir; Britton, Steven L; Koch, Lauren G; Smith, Godfrey L; Wisløff, Ulrik; Høydal, Morten Andre (Peer reviewed, 2013-10-16)
      Background: Although high aerobic capacity is associated with effective cardiac function, the effect of aerobic capacity on atrial function, especially in terms of cellular mechanisms, is not known. We aimed to investigate ...
    • Backbone 1H, 13C and 15N chemical shift assignment of full-length human uracil DNA glycosylase UNG2 

      Buchinger, Edith; Wiik, Siv Åshild; Kusnierczyk, Anna; Rabe, Renana; Aas, Per Arne; Kavli, Bodil Merete; Slupphaug, Geir; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)
      Human uracil N-glycosylase isoform 2—UNG2 consists of an N-terminal intrinsically disordered regulatory domain (UNG2 residues 1–92, 9.3 kDa) and a C-terminal structured catalytic domain (UNG2 residues 93–313, 25.1 kDa). ...
    • Biomarker discovery in multiple myeloma progression by quantitative proteomics 

      Dikic, Aida (Doctoral theses at NTNU;2018:305, Doctoral thesis, 2018)
      SAMMENDRAG Myelomatose (multipel myelom, benmargskreft) er en krefttype som skyldes ukontrollert vekst av plasmaceller i benmarg. Det er den nest vanligste hematologiske kreftformen og rammer ca. 300 personer i Norge hvert ...
    • Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel 

      Petrovic, Voin; Olaisen, Camilla; Sharma, Animesh; Nepal, Anala; Bugge, Steffen; Sundby, Eirik; Hoff, Bård Helge; Slupphaug, Geir; Otterlei, Marit (Journal article; Peer reviewed, 2017)
      Cell extracts from A549, H460, and U2OS human cancer cell lines treated with cisplatin and docetaxel were analyzed by mass spectrometry (MS) proteomic analysis. The extracts were enriched for cellular signaling proteins ...
    • Combining H/D exchange mass spectroscopy and computational docking reveals extended DNA-binding surface on uracil-DNA glycosylase 

      Roberts, Victoria A.; Pique, Michael E.; Hsu, Simon; Li, Sheng; Slupphaug, Geir; Rambo, Robert P.; Jamison, Jonathan P.; Liu, Tong; Lee, Jun H.; Tainer, John A.; Ten Eyck, Lynn F.; Woods Jr., Virgil L. (Journal article; Peer reviewed, 2012)
      X-ray crystallography provides excellent structural data on protein–DNA interfaces, but crystallographic complexes typically contain only small fragments of large DNA molecules. We present a new approach that can use longer ...
    • Differential regulation of cysteine oxidative post-translational modifications in high and low aerobic capacity 

      Souza, Rodrigo W. A.; Alves, Christiano R. R.; Medeiros, Alessandra; Rolim, Natale Pinheiro Lage; Silva, Gustavo Jose Justo; Moreira, Jose Bianco Nascimento; Alves, Marcia Netto Magalhaes; Wohlwend, Martin; Gebriel, Mohammed; Hagen, Lars; Sharma, Animesh; Koch, Lauren G.; Britton, Steven L.; Slupphaug, Geir; Wisløff, Ulrik; Brum, Patricia C. (Journal article; Peer reviewed, 2018)
      Given the association between high aerobic capacity and the prevention of metabolic diseases, elucidating the mechanisms by which high aerobic capacity regulates whole-body metabolic homeostasis is a major research challenge. ...
    • Involvement of the ASC-1 complex in processing of methylated DNA and RNA 

      Rabe, Renana (Doctoral theses at NTNU;2018:83, Doctoral thesis, 2018)
      Den grunnleggende informasjon om organismene ligger lagret i DNA, som er en polymer sammensatt av fire ulike nitrogenbaser bundet til en sukkerfosfat ryggrad. Langs DNA-tråden ligger ulike gener, og baserekkefølgen innen ...
    • Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells. 

      Zub, Kamila Anna; Sousa, Mirta; Sarno, Antonio; Sharma, Animesh; Demirovic, Aida; Rao, Shalini; Young, Clifford; Aas, Per Arne; Ericsson, Ida; Sundan, Anders; Jensen, Ole Nørregaard; Slupphaug, Geir (Journal article; Peer reviewed, 2015)
      Alkylating agents are widely used chemotherapeutics in the treatment of many cancers, including leukemia, lymphoma, multiple myeloma, sarcoma, lung, breast and ovarian cancer. Melphalan is the most commonly used chemotherapeutic ...
    • Multiple Functions of Base Excision Repair Proteins: Relevance to RNA Quality Control and B-Cell Lymphomagenesis 

      Galashevskaya, Anastasia (Doktoravhandlinger ved NTNU, 1503-8181; 2014:325, Doctoral thesis, 2014)
    • Neil3-dependent base excision repair regulates lipid metabolism and prevents atherosclerosis in Apoe-deficient mice 

      Skarpengland, Tonje; Holm, Sverre; Scheffler, Katja; Gregersen, Ida; Dahl, Tuva Børresdatter; Suganthan, Rajikala; Segers, Filip; Østlie, Ingunn; Otten, Jeroen J.T.; Gomez, Luisa Fernanda Luna; Ketelhuth, Daniel F.J.; Lundberg, Anna M.; Neurauter, Christine Gran; Hildrestrand, Gunn Annette; Skjelland, Mona; Bjørndal, Bodil; Svardal, Asbjørn M.; Iversen, Per Ole; Hedin, Ulf; Nygård, Ståle; Olstad, Ole Kristoffer; Krohg-Sørensen, Kirsten; Slupphaug, Geir; Eide, Lars; Kusnierczyk, Anna; Folkersen, Lasse; Ueland, Thor; Berge, Rolf Kristian; Hansson, Göran K.; Biessen, Erik A.L.; Halvorsen, Bente; Bjørås, Magnar; Aukrust, Pål (Peer reviewed; Journal article, 2016)
      Increasing evidence suggests that oxidative DNA damage accumulates in atherosclerosis. Recently, we showed that a genetic variant in the human DNA repair enzyme NEIL3 was associated with increased risk of myocardial ...
    • NEIL3-Dependent Regulation of Cardiac Fibroblast Proliferation Prevents Myocardial Rupture 

      Olsen, Maria Belland; Hildrestrand, Gunn Annette; Scheffler, Katja; Vinge, Leif Erik; Alfsnes, Katrine; Palibrk, Vuk; Wang, Junbai; Neurauter, Christine Gran; Gomez, Luisa Fernanda Luna; Johansen, Jostein; Øgaard, Jonas; Ohm, Ingrid Kristine; Slupphaug, Geir; Kusnierczyk, Anna; Fiane, Arnt E; Brorson, Sverre Henning; Zhang, Lili; Gullestad, Lars; Louch, William Edward; Iversen, Per Ole; Østlie, Ingunn; Klungland, Arne; Christensen, Geir Arve; Sjaastad, Ivar; Sætrom, Pål; Yndestad, Arne; Aukrust, Pål; Bjørås, Magnar; Finsen, Alexandra (Journal article; Peer reviewed, 2017)
      Myocardial infarction (MI) triggers a reparative response involving fibroblast proliferation and differentiation driving extracellular matrix modulation necessary to form a stabilizing scar. Recently, it was shown that a ...
    • No cancer predisposition or increased spontaneous mutation frequencies in NEIL DNA glycosylases-deficient mice 

      Rolseth, Veslemøy; Luna, Luisa; Olsen, Ann-Karin; Suganthan, Rajikala; Scheffler, Katja; Neurauter, Christine Gran; Esbensen, Qin Ying; Kusnierczyk, Anna; Hildrestrand, Gunn Annette; Graupner, Anne; Andersen, Jill Mari; Slupphaug, Geir; Klungland, Arne; Nilsen, Hilde; Bjørås, Magnar (Journal article; Peer reviewed, 2017)
      Base excision repair (BER) is a major pathway for removal of DNA base lesions and maintenance of genomic stability, which is essential in cancer prevention. DNA glycosylases recognize and remove specific lesions in the ...
    • Novel UCHL1 mutations reveal new insights into ubiquitin processing 

      Rydning, Siri Lynne; Backe, Paul Hoff; Sousa, Mirta; Iqbal, Zafar; Øye, Ane-Marte; Sheng, Ying; Yang, Mingyi; Lin, Xiaolin; Slupphaug, Geir; Nordenmark, Tonje Haug; Vigeland, Magnus Dehli; Bjørås, Magnar; Tallaksen, Chantal; Selmer, Kaja Kristine (Journal article; Peer reviewed, 2017)
      Recessive loss of function of the neuronal ubiquitin hydrolase UCHL1 has been implicated in early-onset progressive neurodegeneration (MIM no. 615491), so far only in one family. In this study a second family is characterized, ...
    • Psoriasis pathogenesis – Pso p27 constitutes a compact structure forming large aggregates 

      Lysvand, Hilde; Helland, Ronny; Hagen, Lars; Slupphaug, Geir; Iversen, Ole-Jan (Journal article; Peer reviewed, 2015)
      Psoriasis is a chronic inflammatory skin disease. The absence of microbial organisms as potential causal agents has given rise to the hypothesis that the inflammation is due to an autoimmune reaction. The defined inflamed ...
    • Purification and characterization of an unusual DNA glycosylase in diatoms 

      Andersen, Martin Vejle (Master thesis, 2012)
      DNA damage may be caused by many different agents, including radiation, spontaneous mutations and chemical mutagens. If the damage is left unrepaired, it may result in mutations or cell death. All organisms contain repair ...