Blar i NTNU Open på forfatter "Slupphaug, Geir"
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Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells.
Zub, Kamila Anna; Sousa, Mirta; Sarno, Antonio; Sharma, Animesh; Demirovic, Aida; Rao, Shalini; Young, Clifford; Aas, Per Arne; Ericsson, Ida; Sundan, Anders; Jensen, Ole Nørregaard; Slupphaug, Geir (Journal article; Peer reviewed, 2015)Alkylating agents are widely used chemotherapeutics in the treatment of many cancers, including leukemia, lymphoma, multiple myeloma, sarcoma, lung, breast and ovarian cancer. Melphalan is the most commonly used chemotherapeutic ... -
MS-proteomics provides insight into the host responses towards alginate microspheres
Coron, Abba Elizabeth; Fonseca, Davi de Miranda; Sharma, Animesh; Slupphaug, Geir; Strand, Berit Løkensgard; Rokstad, Anne Mari A. (Journal article; Peer reviewed, 2022)Protein adsorption to biomaterial surfaces is considered a determining factor for the host response. Here we detail the protein adsorption profiles of alginate hydrogel microspheres relevant for cell therapy using mass ... -
Multiple Functions of Base Excision Repair Proteins: Relevance to RNA Quality Control and B-Cell Lymphomagenesis
Galashevskaya, Anastasia (Doktoravhandlinger ved NTNU, 1503-8181; 2014:325, Doctoral thesis, 2014) -
NEIL1 and NEIL2 DNA glycosylases modulate anxiety and learning in a cooperative manner in mice
Hildrestrand, Gunn Annette; Rolseth, Veslemøy; Kunath, Nicolas; Suganthan, Rajikala; Jensen, Vidar; Bugaj, Anna Maria; Fernandez Berrocal, Marion Silvana; SIkko, Sunniva Bøe; Vetlesen, Susanne; Kusnierczyk, Anna; Olsen, Ann-Karin Hardie; Gutzkow, Kristine Bjerve; Rowe, Alexander D.; Wang, Wei; Moldestad, Olve; Syrstad, Monika; Slupphaug, Geir; Eide, Lars; Klungland, Arne; Sætrom, Pål; Luna, Luisa; Ye, Jing; Scheffler, Katja; Bjørås, Magnar (Peer reviewed; Journal article, 2021)Oxidative DNA damage in the brain has been implicated in neurodegeneration and cognitive decline. DNA glycosylases initiate base excision repair (BER), the main pathway for oxidative DNA base lesion repair. NEIL1 and NEIL3 ... -
Neil3-dependent base excision repair regulates lipid metabolism and prevents atherosclerosis in Apoe-deficient mice
Skarpengland, Tonje; Holm, Sverre; Scheffler, Katja; Gregersen, Ida; Dahl, Tuva Børresdatter; Suganthan, Rajikala; Segers, Filip; Østlie, Ingunn; Otten, Jeroen J.T.; Gomez, Luisa Fernanda Luna; Ketelhuth, Daniel F.J.; Lundberg, Anna M.; Neurauter, Christine Gran; Hildrestrand, Gunn Annette; Skjelland, Mona; Bjørndal, Bodil; Svardal, Asbjørn M.; Iversen, Per Ole; Hedin, Ulf; Nygård, Ståle; Olstad, Ole Kristoffer; Krohg-Sørensen, Kirsten; Slupphaug, Geir; Eide, Lars; Kusnierczyk, Anna; Folkersen, Lasse; Ueland, Thor; Berge, Rolf Kristian; Hansson, Göran K.; Biessen, Erik A.L.; Halvorsen, Bente; Bjørås, Magnar; Aukrust, Pål (Peer reviewed; Journal article, 2016)Increasing evidence suggests that oxidative DNA damage accumulates in atherosclerosis. Recently, we showed that a genetic variant in the human DNA repair enzyme NEIL3 was associated with increased risk of myocardial ... -
NEIL3-Dependent Regulation of Cardiac Fibroblast Proliferation Prevents Myocardial Rupture
Olsen, Maria Belland; Hildrestrand, Gunn Annette; Scheffler, Katja; Vinge, Leif Erik; Alfsnes, Katrine; Palibrk, Vuk; Wang, Junbai; Neurauter, Christine Gran; Gomez, Luisa Fernanda Luna; Johansen, Jostein; Øgaard, Jonas; Ohm, Ingrid Kristine; Slupphaug, Geir; Kusnierczyk, Anna; Fiane, Arnt E; Brorson, Sverre Henning; Zhang, Lili; Gullestad, Lars; Louch, William Edward; Iversen, Per Ole; Østlie, Ingunn; Klungland, Arne; Christensen, Geir Arve; Sjaastad, Ivar; Sætrom, Pål; Yndestad, Arne; Aukrust, Pål; Bjørås, Magnar; Finsen, Alexandra (Journal article; Peer reviewed, 2017)Myocardial infarction (MI) triggers a reparative response involving fibroblast proliferation and differentiation driving extracellular matrix modulation necessary to form a stabilizing scar. Recently, it was shown that a ... -
No cancer predisposition or increased spontaneous mutation frequencies in NEIL DNA glycosylases-deficient mice
Rolseth, Veslemøy; Luna, Luisa; Olsen, Ann-Karin; Suganthan, Rajikala; Scheffler, Katja; Neurauter, Christine Gran; Esbensen, Qin Ying; Kusnierczyk, Anna; Hildrestrand, Gunn Annette; Graupner, Anne; Andersen, Jill Mari; Slupphaug, Geir; Klungland, Arne; Nilsen, Hilde; Bjørås, Magnar (Journal article; Peer reviewed, 2017)Base excision repair (BER) is a major pathway for removal of DNA base lesions and maintenance of genomic stability, which is essential in cancer prevention. DNA glycosylases recognize and remove specific lesions in the ... -
Novel UCHL1 mutations reveal new insights into ubiquitin processing
Rydning, Siri Lynne; Backe, Paul Hoff; Sousa, Mirta; Iqbal, Zafar; Øye, Ane-Marte; Sheng, Ying; Yang, Mingyi; Lin, Xiaolin; Slupphaug, Geir; Nordenmark, Tonje Haug; Vigeland, Magnus Dehli; Bjørås, Magnar; Tallaksen, Chantal; Selmer, Kaja Kristine (Journal article; Peer reviewed, 2017)Recessive loss of function of the neuronal ubiquitin hydrolase UCHL1 has been implicated in early-onset progressive neurodegeneration (MIM no. 615491), so far only in one family. In this study a second family is characterized, ... -
Påvirker 5-metylcytosin i det humane genom fjerning av uracil?
Klæstad, Elise (Master thesis, 2020)UNG2 (uracil DNA-glykosylase) er det dominerende enzymet som fjerner uracil i det humane genomet. Metylert cytosin (5-metylcytosin (5-mC)) utgjør omtrent 1% av det humane DNA, og er med det den vanligste epigenetiske ... -
Proteome alterations associated with transformation of multiple myeloma to secondary plasma cell leukemia
Zatula, Alexey; Dikic, Aida; Mulder, Teska Celine; Sharma, Animesh; Vågbø, Cathrine Broberg; Sousa, Mirta; Waage, Anders; Slupphaug, Geir (Peer reviewed; Journal article, 2017)Plasma cell leukemia is a rare and aggressive plasma cell neoplasm that may either originate de novo (primary PCL) or by leukemic transformation of multiple myeloma (MM) to secondary PCL (sPCL). The prognosis of sPCL is ... -
Proteomic analysis reveals mechanisms underlying increased efficacy of bleomycin by photochemical internalization in bladder cancer cells
Gederaas, Odrun Arna; Sharma, Animesh; Mbarak, Saide; Sporsheim, Bjørnar; Høgset, Anders; Bogoeva, Vanya; Slupphaug, Geir; Hagen, Lars (Peer reviewed; Journal article, 2023)Photochemical internalization (PCI) is a promising new technology for site-specific drug delivery, developed from photodynamic therapy (PDT). In PCI, light-induced activation of a photosensitizer trapped inside endosomes ... -
Psoriasis pathogenesis – Pso p27 constitutes a compact structure forming large aggregates
Lysvand, Hilde; Helland, Ronny; Hagen, Lars; Slupphaug, Geir; Iversen, Ole-Jan (Journal article; Peer reviewed, 2015)Psoriasis is a chronic inflammatory skin disease. The absence of microbial organisms as potential causal agents has given rise to the hypothesis that the inflammation is due to an autoimmune reaction. The defined inflamed ... -
Purification and characterization of an unusual DNA glycosylase in diatoms
Andersen, Martin Vejle (Master thesis, 2012)DNA damage may be caused by many different agents, including radiation, spontaneous mutations and chemical mutagens. If the damage is left unrepaired, it may result in mutations or cell death. All organisms contain repair ... -
RNA in DNA repair
Vågbø, Cathrine Broberg; Slupphaug, Geir (Peer reviewed; Journal article, 2020)Our genome is constantly subject to damage from exogenous and endogenous sources, and cells respond to such damage by initiating a DNA damage response (DDR). Failure to induce an adequate DDR can result in increased mutation ... -
RPA2 winged-helix domain facilitates UNG-mediated removal of uracil from ssDNA; implications for repair of mutagenic uracil at the replication fork
Kavli, Bodil Merete; Iveland, Tobias Solli; Buchinger, Edith; Hagen, Lars; Liabakk, Nina-Beate; Aas, Per Arne; Obermann, Tobias Sebastian; Aachmann, Finn Lillelund; Slupphaug, Geir (Journal article; Peer reviewed, 2021)Uracil occurs at replication forks via misincorporation of deoxyuridine monophosphate (dUMP) or via deamination of existing cytosines, which occurs 2-3 orders of magnitude faster in ssDNA than in dsDNA and is 100% miscoding. ... -
Synergistic Actions of Ogg1 and Mutyh DNA Glycosylases Modulate Anxiety-like Behavior in Mice
Bjørge, Monica Dahl; Hildrestrand, Gunn Annette; Scheffler, Katja; Suganthan, Rajikala; Rolseth, Veslemøy; Kusnierczyk, Anna; Rowe, Alexander D.; Vågbø, Cathrine Broberg; Vetlesen, Susanne; Eide, Lars; Slupphaug, Geir; Yusaku, Nakabeppu; Timothy, Bredy; Klungland, Arne; Bjørås, Magnar (Journal article; Peer reviewed, 2015)Ogg1 and Mutyh DNA glycosylases cooperate to prevent mutations caused by 8-oxoG, a major premutagenic DNA lesion associated with cognitive decline. We have examined behavior and cognitive function in mice deficient of these ... -
The human methyltransferase ZCCHC4 catalyses N6-methyladenosine modification of 28S ribosomal RNA
Pinto, Rita; Vågbø, Cathrine Broberg; Jakobsson, Magnus; Yeji, Kim; Baltissen, Marijke P; O'Donohue, Marie-Francoise; Guzman, Ulises H; Malecki, Jedrzej Mieczyslaw; Wu, Jie; Kirpekar, Finn; Olsen, Jesper V; Gleizes, Pierre-Emmanuel; Vermeulen, Michiel; Leidel, Sebastian A.; Slupphaug, Geir; Falnes, Pål Øystein (Journal article; Peer reviewed, 2019)RNA methylations are essential both for RNA structure and function, and are introduced by a number of distinct methyltransferases (MTases). In recent years, N6-methyladenosine (m6A) modification of eukaryotic mRNA has been ... -
UDP-glucose dehydrogenase expression is upregulated following EMT and differentially affects intracellular glycerophosphocholine and acetylaspartate levels in breast mesenchymal cell lines
Wang, Qiong; Karvelsson, Sigurdur Trausti; Johannsson, Freyr; Vilhjalmsson, Arnar Ingi; Hagen, Lars; Fonseca, Davi de Miranda; Sharma, Animesh; Slupphaug, Geir; Rolfsson, Ottar (Journal article; Peer reviewed, 2021)Metabolic rewiring is one of the indispensable drivers of epithelial-mesenchymal transition (EMT) involved in breast cancer metastasis. In this study, we explored the metabolic changes during spontaneous EMT in three ... -
UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation
Pettersen, Henrik Sahlin; Visnes, Torkild; Vågbø, Cathrine Broberg; Svaasand, Eva K; Doseth, Berit; Slupphaug, Geir; Kavli, Bodil Merete; Krokan, Hans Einar (Journal article; Peer reviewed, 2011)Cytotoxicity of 5-fluorouracil (FU) and 5-fluoro-2′-deoxyuridine (FdUrd) due to DNA fragmentation during DNA repair has been proposed as an alternative to effects from thymidylate synthase (TS) inhibition or RNA incorporation. ... -
Uracil DNA Glycosylase UNG2 carrying the germline mutation Arg88Cys: Potential implications for adaptive immunity and cancer
Olaisen, Camilla (Master thesis, 2011)Uracil is normally found in RNA where it replaces the DNA base thymine. However, it can occur in DNA if misincorporated in place of thymine, or as a result of deamination of cytosine. Uracil is also a normal intermediate ...