Browsing NTNU Open by Author "Slupphaug, Geir"

Now showing items 1-20 of 41

    • A targeted mass spectrometry immunoassay to quantify osteopontin in fresh-frozen breast tumors and adjacent normal breast tissues 

      Macur, Katarzyna; Hagen, Lars; Ciesielski, Thomasz M.; Konieczna, Lucyna; Skokowski, Jaroslaw; Jenssen, Bjørn Munro; Slupphaug, Geir; Baczek, Tomasz (Journal article; Peer reviewed, 2019)
      Osteopontin (OPN) is a multifunctional protein that can activate cell-signaling pathways and lead to cancer development and metastasis. Elevated OPN expression was reported in different cancer types, including breast tumors. ...

    • A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair 

      Brickner, Joshua R; Soll, Jennifer M; Lombardi, Patrick M; Vågbø, Cathrine Broberg; Mudge, Miranda C; Oyeniran, Clement; Rabe, Renana; Jackson, Jessica; Sullender, Meagan E; Blazosky, Elyse; Byrum, Andrea K; Zhao, Yu; Corbett, Mark A.; Gècz, Jozef; Field, Michael; Vindigni, Alessandro; Slupphaug, Geir; Wolberger, Cynthia; Mosammaparast, Nima (Journal article; Peer reviewed, 2017)
      DNA repair is essential to prevent the cytotoxic or mutagenic effects of various types of DNA lesions, which are sensed by distinct pathways to recruit repair factors specific to the damage type. Although biochemical ...

    • AID expression in B-cell lymphomas causes accumulation of genomic uracil and a distinct AID mutational signature 

      Pettersen, Henrik Sahlin; Galashevskaya, Anastasia; Doseth, Berit; Sousa, Mirta; Sarno, Antonio; Visnes, Torkild; Aas, Per Arne; Liabakk, Nina-Beate; Slupphaug, Geir; Sætrom, Pål; Kavli, Bodil Merete; Krokan, Hans Einar (Journal article; Peer reviewed, 2014)
      tThe most common mutations in cancer are C to T transitions, but their origin has remained elusive.Recently, mutational signatures of APOBEC-family cytosine deaminases were identified in many com-mon cancers, suggesting ...

    • ALKBH3 partner ASCC3 mediates P-body formation and selective clearance of MMS-induced 1-methyladenosine and 3-methylcytosine from mRNA 

      Wollen, Kristian Lied; Hagen, Lars; Vågbø, Cathrine Broberg; Rabe, Renana; Iveland, Tobias Solli; Aas, Per Arne; Sharma, Animesh; Sporsheim, Bjørnar; Erlandsen, Hilde O.; Palibrk, Vuk; Bjørås, Magnar; Fonseca, Davi de Miranda; Mosammaparast, Nima; Slupphaug, Geir (Peer reviewed; Journal article, 2021)
      Background: Reversible enzymatic methylation of mammalian mRNA is widespread and serves crucial regulatory functions, but little is known to what degree chemical alkylators mediate overlapping modifcations and whether cells ...

    • An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells 

      Sousa, Mirta; Zub, Kamila Anna; Aas, Per Arne; Hanssen-Bauer, Audun; Demirovic, Aida; Sarno, Antonio; Tian, Erming; Liabakk, Nina-Beate; Slupphaug, Geir (Journal article; Peer reviewed, 2013)
      Alterations in checkpoint and DNA repair pathways may provide adaptive mechanisms contributing to acquired drug resistance. Here, we investigated the levels of proteins mediating DNA damage signaling and -repair in RPMI8226 ...

    • Antibody cross-linking and target elution protocols used for immunoprecipitation significantly modulate signal-to noise ratio in downstream 2D-PAGE analysis 

      Sousa, Mirta; Steen, Kristian Wollen; Hagen, Lars; Slupphaug, Geir (Journal article; Peer reviewed, 2011)
      Background Immunoprecipitation and subsequent 2D-PAGE/mass spectrometry are powerful tools to study post-translational protein modifications. Often disregarded in this workflow is the impact of the chemical cross-linker ...

    • Atrial Myocyte Function and Ca2+ Handling Is Associated with Inborn Aerobic Capacity 

      Johnsen, Anne Berit; Rolim, Natale Pinheiro Lage; Stølen, Tomas; Alves, Marcia; Sousa, Mirta; Slupphaug, Geir; Britton, Steven L; Koch, Lauren G; Smith, Godfrey L; Wisløff, Ulrik; Høydal, Morten Andre (Peer reviewed; Journal article, 2013-10-16)
      Background: Although high aerobic capacity is associated with effective cardiac function, the effect of aerobic capacity on atrial function, especially in terms of cellular mechanisms, is not known. We aimed to investigate ...

    • Backbone 1H, 13C and 15N chemical shift assignment of full-length human uracil DNA glycosylase UNG2 

      Buchinger, Edith; Wiik, Siv Åshild; Kusnierczyk, Anna; Rabe, Renana; Aas, Per Arne; Kavli, Bodil Merete; Slupphaug, Geir; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)
      Human uracil N-glycosylase isoform 2—UNG2 consists of an N-terminal intrinsically disordered regulatory domain (UNG2 residues 1–92, 9.3 kDa) and a C-terminal structured catalytic domain (UNG2 residues 93–313, 25.1 kDa). ...

    • Biomarker discovery in multiple myeloma progression by quantitative proteomics 

      Dikic, Aida (Doctoral theses at NTNU;2018:305, Doctoral thesis, 2018)
      SAMMENDRAG Myelomatose (multipel myelom, benmargskreft) er en krefttype som skyldes ukontrollert vekst av plasmaceller i benmarg. Det er den nest vanligste hematologiske kreftformen og rammer ca. 300 personer i Norge hvert ...

    • Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel 

      Petrovic, Voin; Olaisen, Camilla; Sharma, Animesh; Nepal, Anala; Bugge, Steffen; Sundby, Eirik; Hoff, Bård Helge; Slupphaug, Geir; Otterlei, Marit (Journal article; Peer reviewed, 2017)
      Cell extracts from A549, H460, and U2OS human cancer cell lines treated with cisplatin and docetaxel were analyzed by mass spectrometry (MS) proteomic analysis. The extracts were enriched for cellular signaling proteins ...

    • Combining H/D exchange mass spectroscopy and computational docking reveals extended DNA-binding surface on uracil-DNA glycosylase 

      Roberts, Victoria A.; Pique, Michael E.; Hsu, Simon; Li, Sheng; Slupphaug, Geir; Rambo, Robert P.; Jamison, Jonathan P.; Liu, Tong; Lee, Jun H.; Tainer, John A.; Ten Eyck, Lynn F.; Woods Jr., Virgil L. (Journal article; Peer reviewed, 2012)
      X-ray crystallography provides excellent structural data on protein–DNA interfaces, but crystallographic complexes typically contain only small fragments of large DNA molecules. We present a new approach that can use longer ...

    • Conversion of ATP to adenosine by CD39 and CD73 in multiple myeloma can be successfully targeted together with adenosine receptor A2A blockade 

      Yang, Rui; Elsaadi, Samah; Misund, Kristine; Abdollahi, Pegah; Vandsemb, Esten; Moen, Siv Helen; Kusnierczyk, Anna; Slupphaug, Geir; Standal, Therese; Waage, Anders; Slørdahl, Tobias Schmidt; Rø, Torstein Baade; Rustad, Even Holth; Sundan, Anders; Hay, Carl; Cooper, Zachary; Schuller, Alwin G.; Woessner, Richard; Borodowski, Alexandra; Menu, Eline; Børset, Magne; Sponaas, Anne-Marit (Peer reviewed; Journal article, 2020)
      Background PD1/PDL1-directed therapies have been unsuccessful for multiple myeloma (MM), an incurable cancer of plasma cells in the bone marrow (BM). Therefore, other immune checkpoints such as extracellular adenosine and ...

    • Differential regulation of cysteine oxidative post-translational modifications in high and low aerobic capacity 

      Souza, Rodrigo W. A.; Alves, Christiano R. R.; Medeiros, Alessandra; Rolim, Natale Pinheiro Lage; Silva, Gustavo Jose Justo; Moreira, Jose Bianco Nascimento; Alves, Marcia Netto Magalhaes; Wohlwend, Martin; Gebriel, Mohammed; Hagen, Lars; Sharma, Animesh; Koch, Lauren G.; Britton, Steven L.; Slupphaug, Geir; Wisløff, Ulrik; Brum, Patricia C. (Journal article; Peer reviewed, 2018)
      Given the association between high aerobic capacity and the prevention of metabolic diseases, elucidating the mechanisms by which high aerobic capacity regulates whole-body metabolic homeostasis is a major research challenge. ...

    • Exercise training reverses cancer-induced oxidative stress and decrease in muscle COPS2/TRIP15/ALIEN 

      Alves, Christiano R. R.; des Neves, Willian; de Almeida, Ney R; Eichelberger, Eric J; Jannig, Paulo R.; Voltarelli, Vanessa A; Tobias, Gabriel C; Bechara, Luiz R G; de Paula Faria, Daniele; Alves, Maria J N; Hagen, Lars; Sharma, Animesh; Slupphaug, Geir; Moreira, Jose Bianco Nascimento; Wisløff, Ulrik; Hirshman, Michael F.; Negrao, Carlos E.; de Castro Jr, Gilberto; Chammas, Roger; Swoboda, Kathryn J.; Ruas, Jorge L.; Goodyear, Laurie J.; Brum, Patricia Chakur (Peer reviewed; Journal article, 2020)
      Objective We tested the hypothesis that exercise training would attenuate metabolic impairment in a model of severe cancer cachexia. Methods We used multiple in vivo and in vitro methods to explore the mechanisms ...

    • Genomic Uracil and Aberrant Profile of Demethylation Intermediates in Epigenetics and Hematologic Malignancies 

      Olinski, Ryszard; Slupphaug, Geir; Foksinski, Marek; Krokan, Hans Einar (Journal article; Peer reviewed, 2021)
      DNA of all living cells undergoes continuous structural and chemical alterations resulting from fundamental cellular metabolic processes and reactivity of normal cellular metabolites and constituents. Examples include ...

    • HDACi Mediate UNG2 Depletion, Dysregulated Genomic Uracil and Altered Expression of Oncoproteins and Tumor Suppressors in B- And T-cell Lines 

      Iveland, Tobias Solli; Hagen, Lars; Sharma, Animesh; Sousa, Mirta; Sarno, Antonio; Kristian Lied, Wollen; Liabakk, Nina-Beate; Slupphaug, Geir (Peer reviewed; Journal article, 2020)
      Background HDAC inhibitors (HDACi) belong to a new group of chemotherapeutics that are increasingly used in the treatment of lymphocyte-derived malignancies, but their mechanisms of action remain poorly understood. Here ...

    • Impact of HIV-1 Vpr manipulation of the DNA repair enzyme UNG2 on B lymphocyte class switch recombination 

      Eldin, Patrick; Péron, Sophie; Galashevskaya, Anastasia; Denis-Lagache, Nicolas; Cogné, Michel; Slupphaug, Geir; Briant, Laurence (Peer reviewed; Journal article, 2020)
      Background HIV-1 Vpr encodes a 14 kDa protein that has been implicated in viral pathogenesis through modulation of several host cell functions. In addition to pro-apoptotic and cytostatic properties, Vpr can redirect ...

    • Involvement of the ASC-1 complex in processing of methylated DNA and RNA 

      Rabe, Renana (Doctoral theses at NTNU;2018:83, Doctoral thesis, 2018)
      Den grunnleggende informasjon om organismene ligger lagret i DNA, som er en polymer sammensatt av fire ulike nitrogenbaser bundet til en sukkerfosfat ryggrad. Langs DNA-tråden ligger ulike gener, og baserekkefølgen innen ...

    • Long-Term Exposure to Nanosized TiO2 Triggers Stress Responses and Cell Death Pathways in Pulmonary Epithelial Cells 

      Alswady-Hoff, Mayes; Erdem, Johanna Maria Samulin; Phuyal, Santosh; Knittelfelder, Oskar; Sharma, Animesh; Fonseca, Davi de Miranda; Skare, Øivind; Slupphaug, Geir; Zienolddiny, Shanbeh (Peer reviewed; Journal article, 2021)
      There is little in vitro data available on long-term effects of TiO2 exposure. Such data are important for improving the understanding of underlying mechanisms of adverse health effects of TiO2 . Here, we exposed pulmonary ...

    • A loss-of-function mutation in human Oxidation Resistance 1 disrupts the spatial–temporal regulation of histone arginine methylation in neurodevelopment 

      Lin, Xiaolin; Wang, Wei; Yang, Mingyi; Damseh, Nadirah; Sousa, Mirta; Jacob, Fadi; lång, anna ulrika; Kristiansen, Elise; Pannone, Marco; Kissova, Miroslava; Almaas, Runar; Kusnierczyk, Anna; Siller, Richard; Shahrour, Maher; Al-Ashhab, Motee; Abu-Libdeh, Bassam; Tang, Wannan; Slupphaug, Geir; Elpeleg, Orly; Bøe, Stig Ove; Eide, Lars; Sullivan, Gareth John; Rinholm, Johanne Egge; Song, Hongjun; Ming, Guo-li; van Loon, Barbara; Edvardson, Simon; Ye, Jing; Bjørås, Magnar (Peer reviewed; Journal article, 2023)
      Background Oxidation Resistance 1 (OXR1) gene is a highly conserved gene of the TLDc domain-containing family. OXR1 is involved in fundamental biological and cellular processes, including DNA damage response, antioxidant ...