Browsing NTNU Open by Author "Lale, Rahmi"
Now showing items 1-20 of 42
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A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli
Balzer, Simone; Kucharova, Veronika; Megerle, Judith; Lale, Rahmi; Brautaset, Trygve; Valla, Svein (Journal article; Peer reviewed, 2013)Abstract Background Production of recombinant proteins in bacteria for academic and commercial purposes is a well established field; however the outcomes of process developments for specific proteins are still often ... -
A new and improved host-independent plasmid system for RK2-based conjugal transfer
Aakvik, Trine; Lale, Rahmi; Degnes, Kristin Fløgstad; Lando, Malin; Valla, Svein (Journal article; Peer reviewed, 2014)Bacterial conjugation is a process that is mediated either by a direct cell-to-cell junction or by formation of a bridge between the cells. It is often used to transfer DNA constructs designed in Escherichia coli to recipient ... -
Changes in Lipid Composition Induced by Environmental and Genetic Manipulation in Synechocystis sp. PCC 6803
Forfang, Kristin (Master thesis, 2014)The objective of this research was to investigate the photosynthetic lipids synthesized by the cyanobacterium Synechocystis sp. PCC 6803 and to develop methods to extract, separate and characterize them. A particular focus ... -
Characterization and industrial application of artificial promoter and 5' UTR sequences in Escherichia coli and Vibrio natriegens
Mangold, Antonia (Master thesis, 2021)Vibrio natriegens er en gramnegativ proteobakterie som i den siste tiden har fått mye oppmerksomhet på grunn av en rask doblingstid på under ti minutter. Den er fremvok- sende platform for syntetisk biologi, og det er ... -
Cloning and Expression of Four Laccase genes isolated from Arctic marine Psychrobacter strains in Escherichia coli and psychrophilic Pseudomonas
Egharevba, Osasumwen (Master thesis, 2017)A group of diverse bacterial isolates were discovered in Arctic marine habitat during a research expedition in May 2009. These isolates were identified and sequenced based on 16S rRNA sequencing. Psychrobacter strains ... -
Conjugative DNA transfer between bacteria and the heterokont alga Nannochloropsis
Khider, May Laura Kilano (Master thesis, 2018)The heterokont microalgae Nannochloropsis have been the subject of many studies that focus on genomic and metabolic engineering. Research on this genus has been motivated by the algae s high-level production of lipids and ... -
Construction and characterisation of artificial promoters in Bacillus subtilis.
Lykke, Andreas (Master thesis, 2019)Bacillus subtilis en en gram-positiv modellorganisme. Den er mye brukt i både forskning og industri. B. subtilis regnes som en av de viktigste bakteriene for industrien, på grunn av effektivt uttrykk og sekresjon av ... -
Controlling microgel fabrication and advancing understanding of bacterial transcriptional regulation by droplet-based microfluidics
Ahmed, Husnain (Doctoral theses at NTNU;2022:263, Doctoral thesis, 2022)ENGLISH Miniaturization of biological and chemical assays by the invention of Lab-on-a-chip technology has already made significant impacts in scientific domains such as biotechnology, biophysics and biomedicine. Droplet ... -
Design, construction and evaluation of protein expression systems in Synechocystis PCC 6803.
Sunar, Binod Gautam (Master thesis, 2014)student will create constructs that are designed to transfer expression system to the chromosome of Synechocystis. Furthermore it will be tested if the expression systemcan also be implemented on the native plasmids of ... -
Developing molecular tools for the genetic manipulation of Nannochloropsis
Chernyavskaya, Oxana (Master thesis, 2014)The main aim of the master project was the establishment of the efficient protocols for the genetic manipulation (nuclear transformation and chloroplast transformation) of Nannochloropsis oceanica CCMP1779. Nannochloropsis ... -
Development and use of a novel gene expression engineering approach in synthetic biology applications
Tietze, Lisa (Doctoral theses at NTNU;2022:140, Doctoral thesis, 2022)Microorganisms have diverse biochemical capabilities that can be exploited to produce metabolites or proteins of interest, such as drugs, vitamins, or biofuels in industrial scale biomanufacturing processes. To enable ... -
DNA Condensation by Peptide-Conjugated PAMAM Dendrimers. Influence of Peptide Charge
Dannert, Corinna; Mardal, Ingrid; Lale, Rahmi; Stokke, Bjørn Torger; Dias, Rita (Peer reviewed; Journal article, 2023)Nucleic acid delivery to cells is an important therapeutic strategy that requires the transport of nucleic acids to intracellular compartments and their protection from enzymatic degradation. This can be achieved through ... -
Dual UTR-A novel 5′ untranslated region design for synthetic biology applications
Le, Simone Balzer; Onsager, Ingerid; Lorentzen, Jon Andreas; Lale, Rahmi (Peer reviewed; Journal article, 2020)Bacterial 50 untranslated regions of mRNA (UTR) involve in a complex regulation of gene expression; however, the exact sequence features contributing to gene regulation are not yet fully understood. In this study, we report ... -
Effect of design geometry, exposure energy, cytophilic molecules, cell type and load in fabrication of single-cell arrays using micro-contact printing
Bhujbal, Swapnil Vilas; Dekov, Maren; Ottesen, Vegar; Dunker, Karen; Lale, Rahmi; Sletmoen, Marit (Journal article; Peer reviewed, 2020)In this study a range of factors influencing the fabrication of single-cell arrays (SCAs) are identified and investigated. Micro-contact printing was used to introduce spots coated with polyethyleneimine or Matrigel on ... -
Engineering low-temperature expression systems for heterologous production of cold-adapted enzymes
Bjerga, Gro Elin Kjæreng; Lale, Rahmi; Williamson, Adele Kim (Journal article; Peer reviewed, 2016)Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for ... -
Establishment and characterization of artificial promoter and 5′UTR sequences in Escherichia coli and Vibrio natriegens
Hoff, Maria Wilhelmsen (Master thesis, 2020)Forutsigbar og konsekvent regulering av protein produksjon er avgjørende når syntetiske genetiske kretser designes, og ved stor-skala industriell produksjon. For å oppnå dette er det nødvendig å identifisere eller designe ... -
Fluorescent Reporters for investigating the Bacterial Stringent Response
Pahr, Jarle Magnus Ribe (Master thesis, 2013)The ``stringent response'' is a bacterial physiological adaptation to nutritional stress most well studied in the model organism \textit{Escherichia coli}. It is a research topic of interest both for increasing our ... -
Functional Adaptation of Escherichia coli Inducible Promoter Systems for Use in Pseudomonas putida and Bacillus subtilis
Jensen, Magnus Østgård (Master thesis, 2024)Mennesker har brukt mikrober i tusenvis av år, og gjør det nå i større grad enn noen gang før. Anvendelsesområdene er mangfoldige, fra småskala fermentering under hjemmebrygging til storskala industriell biosyntese av ... -
Genome analysis of four novel Psychrobacter spp. and characterisation of their six putative laccase-like multicopper oxidases using bioinformatics tools
Rise, Kjersti (Master thesis, 2015)In May 2009, several Psychrobacter spp. were found at the bottom of the Norwegian sea outside of Svalbard. Four of these, P11F6, P2G3, P11G3 and P11G5, were selected and sequenced for further work, and form the basis for ... -
Genome Mining of actinomycete bacteria for new natural products
Schneider, Olha (Doctoral theses at NTNU;2018:348, Doctoral thesis, 2018)Here, the genome data of seven different strains of Streptomyces and a strain from the Actinoalloteichus genus were analysed for the presence of biosynthetic gene clusters (BGC) encoding for potential new natural products ...