Browsing NTNU Open by Author "Fure, Hilde"
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Air Bubbles Activate Complement and Trigger Hemostasis and C3-Dependent Cytokine Release Ex Vivo in Human Whole Blood
Storm, Benjamin; Christiansen, Dorte; Fure, Hilde; Ludviksen, Judith K; Lau, Corinna; Lambris, John D.; Woodruff, Trent M.; Brekke, Ole-Lars; Braaten, Tonje Bjørndal; Nielsen, Erik Waage; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2021)Venous air embolism, which may complicate medical and surgical procedures, activates complement and triggers thromboinflammation. In lepirudin-anticoagulated human whole blood, we examined the effect of air bubbles on ... -
Alginate microbeads are coagulation compatible, while alginate microcapsules activate coagulation secondary to complement or directly through FXII
Gravastrand, Caroline; Hamad, Shamal; Fure, Hilde; Steinkjer, Bjørg; Ryan, Liv; Oberholzer, José; Lambris, John D.; Lacík, Igor; Mollnes, Tom Eirik; Brekke, Ole-Lars; Rokstad, Anne Mari (Journal article; Peer reviewed, 2017)Alginate microspheres are presently under evaluation for future cell-based therapy. Their ability to induce harmful host reactions needs to be identified for developing the most suitable devices and efficient prevention ... -
Blood Transcriptome Analysis of Septic Patients Reveals a Long Non-Coding Alu-RNA in the Complement C5a Receptor 1 Gene
Emblem, Åse; Knutsen, Erik; Jørgensen, Tor Erik; Fure, Hilde; Johansen, Steinar Daae; Brekke, Ole Lars; Mollnes, Tom Eirik; Karlsen, Bård Ove (Peer reviewed; Journal article, 2022)Many severe inflammation conditions are complement-dependent with the complement component C5a-C5aR1 axis as an important driver. At the RNA level, the blood transcriptome undergoes programmed expression of coding and long ... -
CD14 and complement crosstalk and largely mediate the transcriptional response to Escherichia coli in human whole blood as revealed by DNA microarray
Lau, Corinna; Nygård, Ståle; Fure, Hilde; Olstad, Ole Kristoffer; Holden, Marit; Lappegård, Knut Tore; Brekke, Ole Lars; Espevik, Terje; Hovig, Johannes Eivind; Mollnes, Tom Eirik (Journal article, 2015)Systemic inflammation like in sepsis is still lacking specific diagnostic markers and effective therapeutics. The first line of defense against intruding pathogens and endogenous damage signals is pattern recognition by ... -
Combined inhibition of C5 and CD14 efficiently attenuated the inflammatory response in a porcine model of meningococcal sepsis
Hellerud, Bernt C; Orrem, Hilde L.; Dybwik, Knut Gustav; Pischke, Søren Erik; Barratt-Due, Andreas; Castellheim, Albert; Fure, Hilde; Bergseth, Grete; Christiansen, Dorte; Nunn, Miles A.; Espevik, Terje; Lau, Corinna; Brandtzæg, Petter; Nielsen, Erik Waage; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2017)Background Fulminant meningococcal sepsis, characterized by overwhelming innate immune activation, mostly affects young people and causes high mortality. This study aimed to investigate the effect of targeting two key ... -
Combined inhibition of complement and CD14 improved outcome in porcine polymicrobial sepsis
Skjeflo, Espen Waage; Sagatun, Caroline; Dybwik, Knut; Aam, Sturla; Urving, Sven Haakon; Nunn, Miles A.; Fure, Hilde; Lau, Corinna; Brekke, Ole Lars; Huber-Lang, Markus; Espevik, Terje; Barratt-Due, Andreas; Nielsen, Erik Waage; Mollnes, Tom Eirik (Peer reviewed; Journal article, 2015)Introduction Sepsis is an exaggerated and dysfunctional immune response to infection. Activation of innate immunity recognition systems including complement and the Toll-like receptor family initiate this disproportionate ... -
Complement component 5 does not interfere with physiological hemostasis but is essential for Escherichia coli-induced coagulation accompanied by Toll-like receptor 4.
Landsem, Anne; Fure, Hilde; Ludviksen, Judith K; Christiansen, Dorte; Lau, Corinna; Mathisen, Monica Dammen; Bergseth, Grete; Nymo, Stig Haugset; Lappegård, Knut Tore; Woodruff, T. M.; Espevik, Terje; Mollnes, Tom Eirik; Brekke, Ole-Lars (Journal article; Peer reviewed, 2018)There is a close cross‐talk between complement, Toll‐like receptors (TLRs) and coagulation. The role of the central complement component 5 (C5) in physiological and pathophysiological hemostasis has not, however, been fully ... -
A conformational change of complement C5 is required for thrombin-mediated cleavage, revealed by a novel ex vivo human whole blood model preserving full thrombin activity
Nilsson, Per; Johnson, Christina; Quach, Huy Quang; Macpherson, Alex; Durrant, Oliver; Pischke, Soeren; Fure, Hilde; Landsem, Anne; Bergseth, Grete; Schjalm, Camilla; Haugaard-Kedström, Linda M.; Huber-Lang, Markus; van den Elsen, Jean; Brekke, Ole-Lars; Mollnes, Tom Eirik (Peer reviewed; Journal article, 2021)Thrombin activation of C5 connects thrombosis to inflammation. Complement research in whole blood ex vivo necessitates anticoagulation, which potentially interferes with the inflammatory modulation by thrombin. We challenged ... -
Dental and Periodontal Health in Acute Intermittent Porphyria
Storjord, Elin; Airila-Månsson, Stella; Karlsen, Katarzyna; Madsen, Martin Ragnar Skjerve; Dahl, Jim André; Landsem, Anne; Fure, Hilde; Ludviksen, Judith K; Fjøse, Johannes Østrem; Dickey, Amy K.; Karlsen, Bård Ove; Nielsen, Erik Waage; Mollnes, Tom Eirik; Brekke, Ole Lars (Peer reviewed; Journal article, 2022)In the inherited metabolic disorder acute intermittent porphyria (AIP), high sugar intake prevents porphyric attacks due to the glucose effect and the following high insulin levels that may lower AIP disease activity. ... -
Effect of the anticoagulant, storage time and temperature of blood samples on the concentrations of 27 multiplex assayed cytokines - Consequences for defining reference values in healthy humans
Hennø, Linda Torrissen; Storjord, Elin; Christiansen, Dorte; Bergseth, Grete; Ludviksen, Judith K; Fure, Hilde; Barene, Svein; Nielsen, Erik Waage; Mollnes, Tom Eirik; Brekke, Ole Lars (Journal article; Peer reviewed, 2017)Cytokines are potentially useful biomarkers of sepsis and other inflammatory conditions. Many cytokines can be released by leukocytes and platelets after sampling. The sampling and processing techniques are consequently ... -
Effekten av C1-inhibitor på E.coli- og LPS-indusert "tissue factor" i humant fullblod
Landsem, Anne (Master thesis, 2012)Sepsis er fortsatt en alvorlig tilstand med høy mortalitet, til tross for mange år med intens forskning. Ved sepsis er det en systemisk inflammasjonsreaksjon som involverer komplement, koagulasjons- og kallikrein-kininsystemet. ... -
Gene expression profiling of Gram-negative bacteria-induced inflammation in human whole blood: The role of complement and CD14-mediated innate immune response
Lau, Corinna; Olstad, Ole Kristoffer; Holden, Marit; Nygård, Ståle; Fure, Hilde; Lappegård, Knut Tore; Brekke, Ole Lars; Espevik, Terje; Hovig, Johannes Eivind; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2015)Non-sterile pathogen-induced sepsis and sterile inflammation like in trauma or ischemia–reperfusion injury may both coincide with the life threatening systemic inflammatory response syndrome and multi-organ failure. ... -
Intestinal inflammatory profile shows increase in a diversity of biomarkers in irritable bowel syndrome
Berg, Leif Kyrre; Goll, Rasmus; Fagerli, Erik; Ludviksen, Judith K; Fure, Hilde; Odd Sverre, Moen; Sørbye, Sveinung; Mollnes, Tom Eirik; Florholmen, Jon (Peer reviewed; Journal article, 2020)Background: It has been proposed that irritable bowel syndrome (IBS) is a low-grade mucosal inflammatory disease. Objective: To characterize the intestinal inflammatory profile in IBS patients with or without fructose ... -
Key role of the number of complement receptor 1 on erythrocytes for binding of Escherichia coli to erythrocytes and for leukocyte phagocytosis and oxidative burst in human whole blood
Brekke, Ole-Lars; Christiansen, Dorte; Kisserli, Aymric; Fure, Hilde; Dahl, Jim André; Donvito, Béatrice; Reveil, Brigitte; Ludviksen, Judith K; Tabary, Thierry; Mollnes, Tom Eirik; Cohen, Jacques H.M. (Peer reviewed; Journal article, 2019)Aim To study the role of complement receptor 1 (CR1) for binding of Escherichia coli (E. coli) to erythrocytes, for leukocyte phagocytosis, oxidative burst and complement activation in human whole blood from a CR1 deficient ... -
NHDL, a recombinant VL/VH hybrid antibody control for IgG2/4 antibodies.
Lau, Corinna; McAdam, MB; Bergseth, G; Grevys, Algirdas; Bruun, JA; Ludviksen, Judith K; Fure, Hilde; Espevik, Terje; Moen, A; Andersen, Jan Terje; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2020)The mechanism of action of recombinant IgG2/4 antibodies involves blocking of their target without the induction of effector functions. Examples are eculizumab (Soliris®), which is used clinically to block complement factor ... -
A Novel Porcine Model of Ischemia-Reperfusion Injury After Cross-Clamping the Thoracic Aorta Revealed Substantial Cardiopulmonary, Thromboinflammatory and Biochemical Changes Without Effect of C1-Inhibitor Treatment
Nielsen, Erik Waage; Miller, Yoav; Brekke, Ole Lars; Grond, Joost; Duong, Anh Hoang; Fure, Hilde; Ludviksen, Judith K; Pettersen, Kristin; Reubsaet, Leon; Solberg, Rigmor; Johansen, Harald Thidemann; Mollnes, Tom Eirik (Peer reviewed; Journal article, 2022)Ischemic injury worsens upon return of blood and innate immunity including the complement system play a central role in ischemia-reperfusion injury (IRI) as in thoracic aortic surgery. Complement component1 inhibitor ... -
Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay
Nilsson, Per; Pettersen, Kristin; Oppermann, Martin; Skjeflo, Espen Waage; Fure, Hilde; Christiansen, Dorte; Mollnes, Tom Eirik (Peer reviewed; Journal article, 2021)Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for ... -
Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model
Pischke, Søren; Hestenes, Siv Merete; Johansen, Harald Thidemann; Fure, Hilde; Bugge, Jan F; Espinoza, Andreas Westenvik; Skulstad, Helge; Edvardsen, Thor; Fosse, Erik; Mollnes, Tom Eirik; Halvorsen, Per Steinar; Nielsen, Erik Waage (Journal article; Peer reviewed, 2019)Introduction Right ventricular (RV) myocardial dysfunction is a common feature in septic shock. It can worsen outcome, but the etiology is poorly understood. Pulmonary artery hypertension (PAH) plays a part in the ... -
Staphylococcus aureus-induced complement activation promotes tissue factor-mediated coagulation
Skjeflo, Espen Waage; Christiansen, Dorte; Fure, Hilde; Ludviksen, Judith K; Woodruff, Trent M.; Espevik, Terje; Nielsen, Erik Waage; Brekke, Ole-Lars; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2018)Background There is extensive cross‐talk between the complement system, the Toll‐like receptors (TLRs), and hemostasis. Consumptive coagulopathy is a hallmark of sepsis, and is often mediated through increased tissue ... -
The Role of Complement in Coagulation Activation Induced by Alginate Microspheres in Human Whole Blood
Hamad, Shamal Ahmad (Master thesis, 2013)Diabetes mellitus (DM) is serious disease that affects millions of people worldwide.Type 1 Diabetes (T1D) is an autoimmune disease, caused by destruction of the insulin producing beta cells (ß-cells) of the pancreas. ...