Show simple item record

dc.contributor.authorKorkeamäki, Jannika Timontytär
dc.contributor.authorRashad, Ahmad
dc.contributor.authorBerstad, Kaia
dc.contributor.authorWeber, Florian
dc.contributor.authorSyverud, Kristin
dc.contributor.authorHaugen, Håvard Jostein
dc.contributor.authorMustafa, Kamal Babikeir Elnour
dc.description.abstractWood-derived TEMPO-oxidized cellulose nanofibrils (CNFs) have potential as scaffolding for bone tissue engineering. Although biocompatible, the material lacks osteoconductive and appropriate mechanical properties. Incorporation of nano-hydroxyapatite (nHA) and modification of scaffold preparation methods could improve applicability. In this study, freeze-dried porous scaffolds were prepared using a range of nHA (0, 20, 33, 50%) and CNF compositions. Not only the microarchitecture but also the chemical composition of the scaffolds was studied. Osteoblast-like osteosarcoma derived cells (Saos-2) were cultured on the scaffolds and their responses (viability, attachment, proliferation, and osteogenic phenotype) to the different scaffolds were documented. The results show that incorporation of nHA influenced the microarchitecture, mechanical stiffness and surface properties of the scaffolds. Moreover, biological characterization demonstrated good cell viability in all the groups. However, the increase of nHA concentration beyond 20% does not offer further advantages. It is concluded that the incorporation of 20% nHA resulted in the widest and most biomimetic pore size distribution, increased surface roughness and improved protein adsorption. These changes in material properties enhanced cell spreading and the osteogenic gene expression of osteoblast-like cells seeded on the scaffolds. Moreover, 20% nHA warrants further investigation as a potential scaffolding material for bone tissue engineering.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleBiomimetic Highly Porous Nanocellulose-Nanohydroxyapatite Scaffolds for Bone Tissue Engineeringen_US
dc.title.alternativeBiomimetic Highly Porous Nanocellulose-Nanohydroxyapatite Scaffolds for Bone Tissue Engineeringen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.relation.projectBergens forskningsstiftelse: BFS2018TMT10en_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal